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Literature summary for 1.15.1.1 extracted from

  • Kumar, A.; Kaachra, A.; Bhardwaj, S.; Kumar, S.
    Copper, zinc superoxide dismutase of Curcuma aromatica is a kinetically stable protein (2014), Process Biochem., 49, 1288-1296 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli Curcuma aromatica

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 17% inhibition at 3 mM Curcuma aromatica
DTT 13% inhibition at 2.5 mM Curcuma aromatica
EDTA 15% inhibition at 0.5 mM Curcuma aromatica
guanidinium hydrochloride 40% inhibition at 2 M, 85% at 5 M Curcuma aromatica
H2O2 58% inhibition at 2 mM Curcuma aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis–Menten and Lineweaver–Burk kinetics Curcuma aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a Cu,Zn SOD Curcuma aromatica
Zn2+ a Cu,Zn SOD Curcuma aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Curcuma aromatica
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Curcuma aromatica E6Y8N8 collected at 10:00 am from the experimental farm of the Institute of Himalayan Bioresource Technology at Palampur, Distt. Kangra, India
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis Curcuma aromatica

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Curcuma aromatica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1250
-
purified recombinant His-tagged enzyme, pH 7.8, 37°C, substrate riboflavin Curcuma aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
Curcuma aromatica O2 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 17500, about, sequence calculation, 2 * 18800, about, SDS-PAGE Curcuma aromatica

Synonyms

Synonyms Comment Organism
Ca-Cu,Zn SOD
-
Curcuma aromatica
Cu,Zn superoxide dismutase
-
Curcuma aromatica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Curcuma aromatica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
80 activity range between -10-80°C Curcuma aromatica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70.45
-
midpoint of thermal transition (Tm) Curcuma aromatica
121
-
Ca-Cu Z- SOD retains 50% of the maximum activity after autoclaving, autoclaved Ca-Cu,Zn SOD exhibits 40% of maximum activity of unautoclaved enzyme at 20°C and 30°C. Thermal inactivation kinetics of Ca-Cu,Zn SOD, overview Curcuma aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Curcuma aromatica

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range Curcuma aromatica

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 Ca–Cu,Zn SOD is stable at a wide storage pH ranging from pH 3 to pH 10. After24 h of incubation, the enzyme retains more than 80% of maximal activity in the pH range of pH 3.0-9.0, whereas at pH 10.0 the enzyme loses 36.21% of maximal activity, profile overview Curcuma aromatica