Cloned (Comment) | Organism |
---|---|
gene sor, complementation of the Escherichia coli QC2375 mutant strain | Desulfarculus baarsii |
Protein Variants | Comment | Organism |
---|---|---|
E114A | the mutant shows significantly modified pulse radiolysis kinetics for the protonation process of the first reaction intermediate compared to the wild-type enzyme, mutation results in both a strengthening of the S-Fe bond and an increase in the extent of freeze-trapping of a Fe-peroxo species after treatment with H2O2 by a specific strengthening of the Fe-O bond, spectroscopic mutant analysis, overview | Desulfarculus baarsii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, resonance Raman spectroscopy | Desulfarculus baarsii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the active site consists of an unusual non-heme Fe2+ center in a [His4 Cys1] square pyramidal pentacoordination | Desulfarculus baarsii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + H+ | Desulfarculus baarsii | the enzyme catalyzes the one-electron reduction of O2 to H2O2, providing an antioxidant defense in some bacteria | rubredoxin + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfarculus baarsii | Q46495 | gene sor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + 2 H+ | the active site consists of an unusual non-heme Fe2+ center in a [His4 Cys1] square pyramidal pentacoordination, the reaction procedes via a Fe3+-peroxo intermediate | Desulfarculus baarsii | rubredoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + H+ | the enzyme catalyzes the one-electron reduction of O2 to H2O2, providing an antioxidant defense in some bacteria | Desulfarculus baarsii | rubredoxin + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
reduced rubredoxin | - |
Desulfarculus baarsii |