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Literature summary for 1.15.1.2 extracted from

  • Rodrigues, J.V.; Victor, B.L.; Huber, H.; Saraiva, L.M.; Soares, C.M.; Cabelli, D.E.; Teixeira, M.
    Superoxide reduction by Nanoarchaeum equitans neelaredoxin, an enzyme lacking the highly conserved glutamate iron ligand (2008), J. Biol. Inorg. Chem., 13, 219-228.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain XL2Blue Nanoarchaeum equitans

Protein Variants

Protein Variants Comment Organism
P8E site-directed mutagenesis, pH-induced transition of the mutant is similar to the wild-type enzyme, the reactivity of the N. equitans P8E Nlr mutant towards superoxide measured at different pHs is identical to that of the wild-type protein Nanoarchaeum equitans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the reaction with superoxide proceeds via formation of an intermediate with a second-order rate constant Nanoarchaeum equitans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1Fe-SOR or neelaredoxin class of superoxide reductases Nanoarchaeum equitans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced rubredoxin + superoxide + H+ Nanoarchaeum equitans superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product rubredoxin + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Nanoarchaeum equitans
-
-
-

Reaction

Reaction Comment Organism Reaction ID
superoxide + reduced rubredoxin + 2 H+ = H2O2 + oxidized rubredoxin reaction mechanism involves the diffusion-limited encounter of superoxide with the reduced iron site and concomitant formation of an Fe3+-(hydro)peroxo adduct that, upon protonation, leads to the formation of hydrogen peroxide. By the end of this process, a glutamate residue coordinates the ferric ion, acting as a sixth ligand Nanoarchaeum equitans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced rubredoxin + superoxide + H+
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Nanoarchaeum equitans rubredoxin + H2O2
-
?
reduced rubredoxin + superoxide + H+ superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product Nanoarchaeum equitans rubredoxin + H2O2
-
?

Subunits

Subunits Comment Organism
More three-dimensional modelling of the enzyme Nanoarchaeum equitans

Synonyms

Synonyms Comment Organism
1Fe-SOR
-
Nanoarchaeum equitans
neelaredoxin
-
Nanoarchaeum equitans
Nlr
-
Nanoarchaeum equitans
SOR
-
Nanoarchaeum equitans

Cofactor

Cofactor Comment Organism Structure
reduced rubredoxin
-
Nanoarchaeum equitans