Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain XL2Blue | Nanoarchaeum equitans |
Protein Variants | Comment | Organism |
---|---|---|
P8E | site-directed mutagenesis, pH-induced transition of the mutant is similar to the wild-type enzyme, the reactivity of the N. equitans P8E Nlr mutant towards superoxide measured at different pHs is identical to that of the wild-type protein | Nanoarchaeum equitans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the reaction with superoxide proceeds via formation of an intermediate with a second-order rate constant | Nanoarchaeum equitans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1Fe-SOR or neelaredoxin class of superoxide reductases | Nanoarchaeum equitans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + H+ | Nanoarchaeum equitans | superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product | rubredoxin + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nanoarchaeum equitans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
superoxide + reduced rubredoxin + 2 H+ = H2O2 + oxidized rubredoxin | reaction mechanism involves the diffusion-limited encounter of superoxide with the reduced iron site and concomitant formation of an Fe3+-(hydro)peroxo adduct that, upon protonation, leads to the formation of hydrogen peroxide. By the end of this process, a glutamate residue coordinates the ferric ion, acting as a sixth ligand | Nanoarchaeum equitans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + H+ | - |
Nanoarchaeum equitans | rubredoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + H+ | superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product | Nanoarchaeum equitans | rubredoxin + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional modelling of the enzyme | Nanoarchaeum equitans |
Synonyms | Comment | Organism |
---|---|---|
1Fe-SOR | - |
Nanoarchaeum equitans |
neelaredoxin | - |
Nanoarchaeum equitans |
Nlr | - |
Nanoarchaeum equitans |
SOR | - |
Nanoarchaeum equitans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
reduced rubredoxin | - |
Nanoarchaeum equitans |