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Literature summary for 1.16.1.1 extracted from
- Biochemical and structural properties of a thermostable mercuric ion reductase from Metallosphaera sedula (2015), Front. Bioeng. Biotechnol., 3, 97 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Msed_1241, phylogenetic analysis and tree, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3) | Metallosphaera sedula |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinanat His-tagged enzyme, vapour diffusion hanging drop method, from 0.085 M Tris, pH 8.5, 15% v/v glycerol, 14% w/v PEG 400, 0.19 M LiSO4, and 20 mg/ml protein, 2 weeks, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement using Tn501 MerA, PDB ID 1ZK7, as template, modeling | Metallosphaera sedula |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E317V/F441Y | mutant Tn501 MerA, structure comparison with the wild-type | Metallosphaera sedula |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
Hg2+ | pH 6.7, 70°C, recombinant enzyme | Metallosphaera sedula |  |
| 0.4 | - |
Hg2+ | pH 6.7, 37°C, recombinant enzyme | Metallosphaera sedula | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg2+ + NADPH | Metallosphaera sedula | - |
Hg + NADP+ + H+ | - |
? | |
| Hg2+ + NADPH | Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | - |
Hg + NADP+ + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metallosphaera sedula | A4YG49 | isolated from Pisciarelli Solfatara in Naples, Italy | - |
| Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | A4YG49 | isolated from Pisciarelli Solfatara in Naples, Italy | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strain BL21(DE3) | Metallosphaera sedula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Metallosphaera sedula is isolated from Pisciarelli Solfatara in Naples, Italy. Pisciarelli Solfatara contains a variety of thermal features that range in temperature from about 30°C to nearly 100°C, and a pH range of pH 1.5 to around pH 6.0 with elevated concentrations of heavy metals, including Hg2+ at concentrations up to 0.005g/kg | Metallosphaera sedula | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | - |
NADPH oxidation-linked Hg2+ reduction specific activity, purified recombinant His-tagged enzyme, pH 6.7, 37°C | Metallosphaera sedula |
| 3.1 | - |
NADPH oxidation-linked Hg2+ reduction specific activity, purified recombinant His-tagged enzyme, pH 6.7, 70°C | Metallosphaera sedula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg2+ + NADPH | - |
Metallosphaera sedula | Hg + NADP+ + H+ | - |
? | |
| Hg2+ + NADPH | - |
Metallosphaera sedula ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2 | Hg + NADP+ + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure comparison of wild-type with mutant Tn501 MerA, PDB ID 1ZK7 | Metallosphaera sedula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MerA | - |
Metallosphaera sedula |
| mercuric ion reductase | - |
Metallosphaera sedula |
| Msed_1241 | - |
Metallosphaera sedula |
| MseMerA | - |
Metallosphaera sedula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | assay at, enzyme activity at 70°C is about 1.6fold higher compared to activity at 37°C | Metallosphaera sedula |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 97 | - |
purified recombinant His-tagged enzyme, pH 6.7, 100 min, activity remaining | Metallosphaera sedula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
assay at | Metallosphaera sedula |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Metallosphaera sedula | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | MerA is part of the disulfide oxidoreductase (DSOR) family, are ancient enzymes that have arisen in high temperature environments after the great oxidation event about 2.4 billion years ago | Metallosphaera sedula |
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