Literature summary for 1.17.1.1 extracted from

Gonzalez-Porque, P.
Cytidine diphosphate 4-keto-6-deoxy-D-glucose-3-dehydrogenase (1986), Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York, 1, 391-419.

No PubMed abstract available

Inhibitors

Inhibitors	Comment	Organism	Structure
5,5'-dithiobis(2-nitrobenzoate)	inhibits the activity of the whole system	Yersinia pseudotuberculosis
N-ethylmaleimide	inhibits the activity of the whole system	Yersinia pseudotuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	cofactor: 200-400, gel filtration on Bio Gel P2	Yersinia pseudotuberculosis
35000	45000	enzyme E3, thin-layer chromatography on Sephadex G-100	Yersinia pseudotuberculosis
40000	-	enzyme E3: 1 * 40000, SDS-PAGE, enzyme E1: 1 * 61000, SDS-PAGE, 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone	Yersinia pseudotuberculosis
60000	70000	enzyme E1, thin-layer chromatography on Sephadex G-100	Yersinia pseudotuberculosis
61000	-	enzyme E3: 1 * 40000, SDS-PAGE, enzyme E1: 1 * 61000, SDS-PAGE, 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone	Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H	Yersinia pseudotuberculosis	biosynthesis of 3,6-dideoxysugars	CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Yersinia pseudotuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
of cofactor, using streptomycin sulfate precipitation, ammonium sulfate precipitation and dialysis, DEAE-cellulose chromatography, ultrafiltration, Bio Gel P-2 filtration and ion exchange chromatography on Dowex-1	Yersinia pseudotuberculosis
the first three steps are common to the purification of enzyme E1, enzyme E3 and cofactor, their separation can be accomplished after step 4, step 1: preparation of the crude extract, step 2: streptomycin sulfate precipitation, step 3: ammonium sulfate precipitation and dialysis, step 4: DEAE-cellulose chromatography, step 5: purification of enzyme E1, gel filtration on Sephadex G-100, step 6: second DEAE-cellulose chromatography, step 7: preparative polyacrylamide gel electrophoresis, step 5': purification of enzyme E3, gel filtration on Sephadex G-100, step 6': second DEAE-cellulose chromatography, step 7': third DEAE-cellulose chromatography	Yersinia pseudotuberculosis

Purification (Comment)

Organism

of cofactor, using streptomycin sulfate precipitation, ammonium sulfate precipitation and dialysis, DEAE-cellulose chromatography, ultrafiltration, Bio Gel P-2 filtration and ion exchange chromatography on Dowex-1

Yersinia pseudotuberculosis

the first three steps are common to the purification of enzyme E1, enzyme E3 and cofactor, their separation can be accomplished after step 4, step 1: preparation of the crude extract, step 2: streptomycin sulfate precipitation, step 3: ammonium sulfate precipitation and dialysis, step 4: DEAE-cellulose chromatography, step 5: purification of enzyme E1, gel filtration on Sephadex G-100, step 6: second DEAE-cellulose chromatography, step 7: preparative polyacrylamide gel electrophoresis, step 5': purification of enzyme E3, gel filtration on Sephadex G-100, step 6': second DEAE-cellulose chromatography, step 7': third DEAE-cellulose chromatography

Yersinia pseudotuberculosis

Reaction

Reaction	Comment	Organism	Reaction ID
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+	mechanism	Yersinia pseudotuberculosis	a
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+	2 proteins, E1 and E3, are involved but no partial reaction is observed in the presence of either alone	Yersinia pseudotuberculosis	a
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+	role of the enzymes E1 and E3, as well as role of the cofactor. Multicomponent system consisting of substrate, NADH or NADPH, enzyme E1: recognition unit of the system by binding to the substrate through the amino group of the coenzyme, enzyme E3: acts as the dehydrogenase of the system, and cofactor: pyridoxamine 5'-phosphate	Yersinia pseudotuberculosis	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.11	-	E1	Yersinia pseudotuberculosis
0.32	-	E3	Yersinia pseudotuberculosis

Storage Stability

Storage Stability	Organism
frozen, for months without loss of activity, enzyme E1	Yersinia pseudotuberculosis
lyophilized powder: enzyme E3 not stable	Yersinia pseudotuberculosis
lyophilized powder: for months, enzyme E1	Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H	the substrate undergoes substitution of a hydroxyl group by a hydrogen atom at carbon atom 3 from glucose, it is supposed that the reaction is triggered by an attack from a hydride ion from NAD(P)H to atom carbon 3	Yersinia pseudotuberculosis	CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O	-	?
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H	biosynthesis of 3,6-dideoxysugars	Yersinia pseudotuberculosis	CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	enzyme E3: 1 * 40000, SDS-PAGE, enzyme E1: 1 * 61000, SDS-PAGE, 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone	Yersinia pseudotuberculosis

Synonyms

Synonyms	Comment	Organism
More	2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone	Yersinia pseudotuberculosis

Cofactor

Cofactor	Comment	Organism
additional information	3.8 SH groups per molecule of enzyme E1, in the presence and absence of SDS	Yersinia pseudotuberculosis
additional information	0.37 SH groups per molecule of enzyme E3, in the absence of SDS and 1 SH group per molecule of enzyme E3 in the presence of SDS, the single SH group in enzyme E3 is essential for activity	Yersinia pseudotuberculosis
additional information	derivatives of vitamin B6 such as pyridoxine, pyridoxine 5'-phosphate, pyridoxal, pyridoxal 5'-phosphate, pyridoxamine and pyridoxamine 5'-phosphate have stimulatory effect on the reaction, indicating that the amino group and the phosphate group are necessary for the reaction	Yersinia pseudotuberculosis
NADH	the second substrate of the reaction, the hydrogen donor	Yersinia pseudotuberculosis
NADPH	the second substrate of the reaction, the hydrogen donor	Yersinia pseudotuberculosis
pyridoxamine 5'-phosphate	bound to enzyme E1 through an ionic interaction with a positive charge on the surface of the enzyme, the cofactor is needed for the binding of the substrate to the enzyme	Yersinia pseudotuberculosis