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Literature summary for 1.17.1.4 extracted from

  • Della Corte, E.; Stirpe, F.
    The regulation of rat liver xanthine oxidase. Involvement of thiol groups in the conversion of the enzyme activity from dehydrogenase (type D) into oxidase (type O) and purification of the enzyme (1972), Biochem. J., 126, 739-745.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.4

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol recovery from oxidase to dehydrogenase type Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis-(2-nitrobenzoate) conversion of dehydrogenase type D to oxidase type O, can be prevented and reversed by dithioerythritol Rattus sp.
CuSO4 conversion of the dehydrogenase type D to oxidase type O, prolonged incubation leads to complete inactivation, conversion can be reversed and prevented by dithioerythritol Rattus sp.
N-ethylmaleimide conversion of dehydrogenase type D to oxidase type O, prevented by dithioerythritol but no reversible conversion Rattus sp.
Tetraethylthiuram disulfide conversion of dehydrogenase type D to oxidase type O, can be prevented and reversed by dithioerythritol Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + NAD+ + H2O Rattus sp.
-
 urate + NADH
-
 ?
xanthine + NAD+ + H2O Gallus gallus only dehydrogenase type D present urate + NADH
-
 ?
xanthine + NAD+ + O2 + H2O + H+ Rattus sp. intermediate form of dehydrogenase/oxidase type D/O urate + NADH + H2O2
-
 ?
xanthine + O2 + H2O Gallus gallus
-
 urate + O2- + 2 H+
-
 ?
xanthine + O2 + H2O Rattus sp.
-
 urate + O2- + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 chicken
-
no activity in Columba livia
-
-
-
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
during purification conversion from dehydrogenase type D to oxidase type O, reversible by dithioerythritol Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
heart only oxidase type O form Rattus sp.
-
intestine only oxidase type O form, can be converted to dehydrogenase type D by dithioerythritol Rattus sp.
-
kidney only oxidase type O form Rattus sp.
-
liver
-
 Gallus gallus
-
liver dehydrogenase and oxidase forms Rattus sp.
-
lung only oxidase type O form Rattus sp.
-
pancreas only oxidase type O form Rattus sp.
-
spleen only oxidase type O form Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthine + NAD+ + H2O
-
 Rattus sp. urate + NADH
-
 ?
xanthine + NAD+ + H2O only dehydrogenase type D present Gallus gallus urate + NADH
-
 ?
xanthine + NAD+ + O2 + H2O + H+ intermediate form of dehydrogenase/oxidase type D/O Rattus sp. urate + NADH + H2O2
-
 ?
xanthine + O2 + H2O
-
 Gallus gallus urate + O2- + 2 H+
-
 ?
xanthine + O2 + H2O
-
 Rattus sp. urate + O2- + 2 H+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
 conversion of dehydrogenase type D to oxidase type O Rattus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gallus gallus
NAD+
-
 Rattus sp.