Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuriphenyl sulfonic acid | 98% inhibition at 0.001 mM | Pseudomonas synxantha | |
Ag2+ | complete inhibition of hypoxanthine oxidation at 0.01 mM | Pseudomonas synxantha | |
Cu2+ | 14% inhibition of hypoxanthine oxidation at 0.1 mM | Pseudomonas synxantha | |
Hg2+ | complete inhibition of hypoxanthine oxidation at 0.1 mM | Pseudomonas synxantha | |
KCN | 37% inhibition at 1 mM | Pseudomonas synxantha |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000 + x * 76000, SDS-PAGE | Pseudomonas synxantha |
76000 | - |
x * 54000 + x * 76000, SDS-PAGE | Pseudomonas synxantha |
540000 | - |
gel filtration | Pseudomonas synxantha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | Pseudomonas synxantha | preferred substrate | xanthine + NADH + H+ | - |
? | |
purine + NAD+ + H2O | Pseudomonas synxantha | poor substrate | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | Pseudomonas synxantha | 75% of the activity compared to hypoxanthine | urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas synxantha | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to crystalline state, chromatography techniques | Pseudomonas synxantha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas synxantha | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Pseudomonas synxantha |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dithiopurine + NAD+ + H2O | 26% of the activity compared to hypoxanthine | Pseudomonas synxantha | ? + NADH | - |
? | |
2-thioxanthine + NAD+ + H2O | 57% of the activity compared to hypoxanthine | Pseudomonas synxantha | 2-thiourate + NADH | - |
? | |
6-thioxanthine + NAD+ + H2O | 63% of the activity compared to hypoxanthine | Pseudomonas synxantha | 6-thiourate + NADH | - |
? | |
8-azahypoxanthine + NAD+ + H2O | 42% of the activity compared to hypoxanthine | Pseudomonas synxantha | 8-azaxanthine + NADH | - |
? | |
adenine-N1-oxide + NAD+ + H2O | 3.4% of the activity compared to hypoxanthine | Pseudomonas synxantha | ? + NADH | - |
? | |
hypoxanthine + 2,6-dichlorophenolindophenol + H2O | considerable activity | Pseudomonas synxantha | xanthine + ? | - |
? | |
hypoxanthine + NAD+ + H2O | preferred substrate | Pseudomonas synxantha | xanthine + NADH + H+ | - |
? | |
hypoxanthine + phenazine methosulfate + H2O | low activity | Pseudomonas synxantha | urate + ? | - |
? | |
isoguanine + NAD+ + H2O | 7.3% of the activity compared to hypoxanthine | Pseudomonas synxantha | ? + NADH | - |
? | |
purine + NAD+ + H2O | poor substrate | Pseudomonas synxantha | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | 75% of the activity compared to hypoxanthine | Pseudomonas synxantha | urate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000 + x * 76000, SDS-PAGE | Pseudomonas synxantha |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas synxantha |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for 10 min, pH 8.5 | Pseudomonas synxantha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | - |
Pseudomonas synxantha |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas synxantha | |
NAD+ | cannot be replaced by NADP+ | Pseudomonas synxantha |