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Literature summary for 1.17.1.4 extracted from
- The Escherichia coli periplasmic aldehyde oxidoreductase is an exceptional member of the xanthine oxidase family of molybdoenzymes (2016), ACS Chem. Biol., 11, 2923-2935.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 1.7 A resolution. The active site of PaoABC is highly exposed to the surface with no aromatic residues and an arginine (subunit PaoC-R440) making a direct interaction with subunit PaoC-E692, which acts as a base catalyst | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R40K | mutation in subunit PaoC, strong decrease in activity | Escherichia coli |
| R440H | mutation in subunit PaoC, strong decrease in activity, crystallization data | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli |  |
| 0.129 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
| 0.159 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
| 0.204 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
| 0.288 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
| 0.307 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
| 0.361 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
| 0.396 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
| 0.514 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
| 0.52 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
| 0.659 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli | |
| 1.517 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | molybdopterin-cytosine-dinucleotide localized in subunit PaoC | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77165 and P77324 and P77489 | P77165 i.e. alpha-subunit, P77324 i.e. beta-subnit, P77489 i.e. gamma-subunit | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + ferricyanide + H2O | - |
Escherichia coli | benzoate + 2 ferrocyanide + 2 H+ | - |
? | |
| cinnamaldehyde + H2O + acceptor | - |
Escherichia coli | cinnamic acid + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | crystallization data | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PaoABC | - |
Escherichia coli |
| YagR | - |
Escherichia coli |
| YagS | - |
Escherichia coli |
| YagT | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
| 48.7 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli | |
| 80.5 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
| 150 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
| 150 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
| 217 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
| 293 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
| 307 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli | |
| 325 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
| 404 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
| 486 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
| 1097 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | subunit PaoB exhibits a typical FAD binding motif | Escherichia coli | |
| molybdopterin cytosine dinucleotide | localized in subunit PaoC | Escherichia coli | |
| [2Fe-2S]-center | iron-sulfur subunit PaoA can be divided into two subdomains, each carrying one [2Fe-2S] cluster | Escherichia coli | |
| [4Fe-4S]-center | cluster is anchored to an additional 40 residues subdomain of subunit PaoB | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 200 | - |
benzaldehyde | mutant R440K, pH 4.0, 22°C | Escherichia coli | |
| 200 | - |
benzaldehyde | mutant R440K, pH 5.5, 22°C | Escherichia coli | |
| 220 | - |
benzaldehyde | mutant R440K, pH 5.0, 22°C | Escherichia coli | |
| 520 | - |
benzaldehyde | mutant R440H, pH 5.0, 22°C | Escherichia coli | |
| 620 | - |
benzaldehyde | mutant R440K, pH 4.5, 22°C | Escherichia coli | |
| 740 | - |
benzaldehyde | mutant R440H, pH 4.5, 22°C | Escherichia coli | |
| 790 | - |
benzaldehyde | mutant R440H, pH 4.0, 22°C | Escherichia coli | |
| 810 | - |
benzaldehyde | mutant R440H, pH 5.5, 22°C | Escherichia coli | |
| 1170 | - |
benzaldehyde | wild-type, pH 5.5, 22°C | Escherichia coli | |
| 1370 | - |
benzaldehyde | wild-type, pH 5.0, 22°C | Escherichia coli | |
| 1580 | - |
benzaldehyde | wild-type, pH 4.5, 22°C | Escherichia coli | |
| 1660 | - |
benzaldehyde | wild-type, pH 4.0, 22°C | Escherichia coli | |
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