Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
methylotrophic bacterium | |
Dithionitrobenzoate | - |
methylotrophic bacterium | |
iodoacetamide | - |
methylotrophic bacterium | |
N3- | - |
methylotrophic bacterium | |
PCMB | - |
methylotrophic bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
NAD+ | - |
methylotrophic bacterium | |
15 | - |
formate | - |
methylotrophic bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | methylotrophic bacterium |
70000 | - |
gel filtration | methylotrophic bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
methylotrophic bacterium | - |
- |
- |
methylotrophic bacterium 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
methylotrophic bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
methylotrophic bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
methylotrophic bacterium | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
methylotrophic bacterium 1 | CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | methylotrophic bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
almost complete inactivation in 2-3 days, -SH compounds protect against inactivation, NAD+ or NADH decreases the inactivation rate, inactivation rate is significantly diminished in presence of formate, strongest stabilization by EDTA | methylotrophic bacterium |
52 | - |
inactivation rate constant is zero | methylotrophic bacterium |
61 | - |
inactivation rate constant: 0.0114/min | methylotrophic bacterium |
65.5 | - |
inactivation rate constant: 0.10/min | methylotrophic bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 10.5 | - |
methylotrophic bacterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
methylotrophic bacterium |