Literature summary for 1.17.1.9 extracted from

Hoepner, T.; Ruschig, U.; Mueller, U.; Willnow, P.
Formate dehydrogenase from Pseudomonas oxalaticus (1982), Methods Enzymol., 89, 531-537.

Search for more literature for 1.17.1.9

Inhibitors

Inhibitors	Comment	Organism	Structure
CN-	-	Cupriavidus oxalaticus
Hg2+	-	Cupriavidus oxalaticus
N3-	0.25 mM. Inhibition is partly overcome by addition of formate or NAD+	Cupriavidus oxalaticus
NADH	inhibition is partly overcome by addition of formate or NAD+	Cupriavidus oxalaticus
NO3-	0.25 mM. Inhibition is partly overcome by addition of formate or NAD+	Cupriavidus oxalaticus
p-hydroxymercuribenzoate	-	Cupriavidus oxalaticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.105	-	NAD+	formate dehydrogenase I, reaction with formate	Cupriavidus oxalaticus
0.11	-	NAD+	formate dehydrogenase II, reaction with formate	Cupriavidus oxalaticus
0.13	-	formate	formate dehydrogenase II, reaction with NAD+	Cupriavidus oxalaticus
0.135	-	formate	formate dehydrogenase I, reaction with NAD+	Cupriavidus oxalaticus
0.2	-	2,6-dichlorophenolindophenol	formate dehydrogenase I or formate dehydrogenase II, reaction with NADH	Cupriavidus oxalaticus
0.24	-	benzyl viologen	formate dehydrogenase II, reaction with formate	Cupriavidus oxalaticus
0.25	-	benzyl viologen	formate dehydrogenase I, reaction with formate	Cupriavidus oxalaticus
0.46	-	ferricyanide	formate dehydrogenase I, reaction with formate	Cupriavidus oxalaticus
0.5	-	ferricyanide	formate dehydrogenase II, reaction with formate	Cupriavidus oxalaticus
0.62	-	ferricyanide	formate dehydrogenase II, reaction with NADH	Cupriavidus oxalaticus
0.69	-	ferricyanide	formate dehydrogenase I, reaction with NADH	Cupriavidus oxalaticus
30	50	CO2	-	Cupriavidus oxalaticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	formate dehydrogenase I contains 18-25 mol of iron per mol of enzyme. Formate dehydrogenase II contains 6-10 mol of iron per mol of enzyme	Cupriavidus oxalaticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
formate + NAD+	Cupriavidus oxalaticus	when Pseudomonas oxalaticus is grown on formate as main carbon and energy source, formate dehydrogenase is the key enzyme that generates NADH and CO	CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus oxalaticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
essential conditions of purification are exclusion of light and oxygen	Cupriavidus oxalaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32	-	-	Cupriavidus oxalaticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + 2,6-dichlorophenolindophenol	-	Cupriavidus oxalaticus	CO2 + reduced 2,6-dichlorophenolindophenol	-	r
formate + FAD	-	Cupriavidus oxalaticus	CO2 + FADH2	-	?
formate + FMN	-	Cupriavidus oxalaticus	CO2 + FMNH2	-	?
formate + methyl viologen	-	Cupriavidus oxalaticus	CO2 + reduced methyl viologen	-	?
formate + methylene blue	-	Cupriavidus oxalaticus	CO2 + reduced methylene blue	-	?
formate + NAD+	-	Cupriavidus oxalaticus	CO2 + NADH + H+	-	r
formate + NAD+	when Pseudomonas oxalaticus is grown on formate as main carbon and energy source, formate dehydrogenase is the key enzyme that generates NADH and CO	Cupriavidus oxalaticus	CO2 + NADH + H+	-	?
formate + nitrobluetetrazolium	-	Cupriavidus oxalaticus	CO2 + reduced nitrobluetetrazolium	-	?
formate + O2	-	Cupriavidus oxalaticus	CO2 + H2O2	-	?
formate + phenazine methosulfate	-	Cupriavidus oxalaticus	CO2 + reduced phenazine methosulfate	-	?
formate + riboflavin	-	Cupriavidus oxalaticus	CO2 + reduced riboflavin	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	carbonate reduction by enzyme I	Cupriavidus oxalaticus
7.4	-	formate dehydrogenation, enzyme I	Cupriavidus oxalaticus

Cofactor

Cofactor	Comment	Organism	Structure
FMN	formate dehydrogenase I contains 1 mol of FMN per mol of enzyme, formate dehydrogenase II contains 2 mol of FMN per mol of enzyme	Cupriavidus oxalaticus
NAD+	-	Cupriavidus oxalaticus

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Release 2023.1 (January 2023)