Literature summary for 1.17.1.9 extracted from

Duarte, R.O.; Reis, A.R.; Girio, F.; Moura, I.; Moura, J.J.G.; Collaco, T.A.
The formate dehydrogenase isolated from the aerobe Methylobacterium sp. RXM is a molybdenum-containing protein (1997), Biochem. Biophys. Res. Commun., 230, 30-34.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Methylobacterium sp.
imidazole	-	Methylobacterium sp.
N3-	0.1 mM	Methylobacterium sp.
PCMB	-	Methylobacterium sp.
Zn2+	-	Methylobacterium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.7	-	formate	-	Methylobacterium sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	two distinct [Fe-S] centres	Methylobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	4 * 75000, SDS-PAGE	Methylobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Methylobacterium sp.	-	RXM	-
Methylobacterium sp. RXM	-	RXM	-

Oxidation Stability

Oxidation Stability	Organism
unstable under oxygen	Methylobacterium sp.

Purification (Commentary)

Purification (Comment)	Organism
partial	Methylobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8	-	-	Methylobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	Methylobacterium sp.	CO2 + NADH + H+	-	?
formate + NAD+	-	Methylobacterium sp. RXM	CO2 + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 75000, SDS-PAGE	Methylobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	-	10 min, stable	Methylobacterium sp.
65	-	10 min, complete inactivation	Methylobacterium sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Methylobacterium sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.5	10	10 min, stable	Methylobacterium sp.

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Methylobacterium sp.

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Release 2023.1 (January 2023)