Protein Variants | Comment | Organism |
---|---|---|
D195S | mutant enzyme also can use NADP+ as coenzyme | [Candida] methylica |
D195S | mutant enzyme shows similar catalytic constant to wild-type in the reaction with NAD+. In contrast with wild-type, the reaction of NADP+ catalyzed by the mutant is clearly discernible | [Candida] methylica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
NAD+ | mutant enzyme D195S | [Candida] methylica | |
4.7 | - |
NAD+ | pH 8.0, 20°C, mutant enzyme D195S | [Candida] methylica | |
5.5 | - |
NAD+ | wild-type enzyme | [Candida] methylica | |
5.5 | - |
NAD+ | pH 8.0, 20°C, wild-type enzyme | [Candida] methylica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | [Candida] methylica | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] methylica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme D195S | [Candida] methylica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] methylica | CO2 + NADH + H+ | - |
? | |
formate + NADP+ | no reaction is catalyzed with wild-type enzyme, activity is detected with mutant enzyme D195S. The ratio of the catalytic efficiencies for NAD+ versus NADP+ for the mutant protein is 40:1 in favor of NAD+ | [Candida] methylica | CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD-specific formate dehydrogenase | - |
[Candida] methylica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
NAD+ | wild-type enzyme | [Candida] methylica | |
1.4 | - |
NAD+ | pH 8.0, 20°C, wild-type enzyme | [Candida] methylica | |
1.6 | - |
NAD+ | mutant enzyme S195S | [Candida] methylica | |
1.6 | - |
NAD+ | pH 8.0, 20°C, mutant enzyme D195S | [Candida] methylica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] methylica | |
NADP+ | no reaction is catalyzed with wild-type enzyme, activity is detected with mutant enzyme D195S. The ratio of the catalytic efficiencies for NAD+ versus NADP+ for the mutant protein is 40:1 in favor of NAD+ | [Candida] methylica |