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Literature summary for 1.17.1.9 extracted from

  • Watanabe, T.; Hattori, T.; Tengku, S.; Shimada, M.
    Purification and characterization of NAD-dependent formate dehydrogenase from the white-rot fungus Ceriporiopsis subvermispora and a possible role of the enzyme in oxalate metabolism (2005), Enzyme Microb. Technol., 37, 68-75.
No PubMed abstract available
Search for more literature for 1.17.1.9

Inhibitors

Inhibitors Comment Organism Structure
ADP-beta-D-ribose competitive with NAD+, mixed-type with formate Gelatoporia subvermispora
azide uncompetitive with NAD+, competitive with formate Gelatoporia subvermispora
bicarbonate mixed-type inhibition of NAD+ and formate Gelatoporia subvermispora
Hg2+ strong inhibition Gelatoporia subvermispora
iodoacetate strong inhibition Gelatoporia subvermispora
additional information not inhibitory: Mg2+, Mn2+, Co2+, Ni2+, Cd2+, Zn2+, Ca2+, Fe2+, Fe3+, Cu2+ Gelatoporia subvermispora
NADH competitive with NADH, mixed-type with formate Gelatoporia subvermispora
NADP+ strong inhibition Gelatoporia subvermispora
NADPH strong inhibition Gelatoporia subvermispora
p-chloromercuribenzoate strong inhibition Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 NAD+ pH 6.5, 30°C Gelatoporia subvermispora
2.5
-
 formate pH 6.5, 30°C Gelatoporia subvermispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Gelatoporia subvermispora
84000
-
 gel filtration Gelatoporia subvermispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gelatoporia subvermispora possible role of enzyme in oxalate metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gelatoporia subvermispora

Reaction

Reaction Comment Organism Reaction ID
formate + NAD+ = CO2 + NADH + H+ ordered BiBi mechanism with NAD+ and NADH being the first substrate and the second product, resp. Gelatoporia subvermispora
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
383.4
-
 pH 6.5, 30°C Gelatoporia subvermispora

Storage Stability

Storage Stability Organism
activity is retained in presence of dithiothreitol for 6 months, but lost within weeks in absence of SH compounds Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Gelatoporia subvermispora CO2 + NADH + H+
-
 ?
additional information possible role of enzyme in oxalate metabolism Gelatoporia subvermispora ?
-
 ?
additional information no substrate: acetate, pyruvate, malate, oxalate, succinate, methanol, isocitrate, fumarate Gelatoporia subvermispora ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Gelatoporia subvermispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Gelatoporia subvermispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Gelatoporia subvermispora

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for NAD+ as electron acceptor Gelatoporia subvermispora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000239
-
 azide substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
0.00095
-
 azide substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
0.0228
-
 ADP-beta-D-ribose substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
0.0369
-
 ADP-beta-D-ribose substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
0.053
-
 bicarbonate substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
0.06
-
 NADH substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora
1.2
-
 bicarbonate substrate formate, pH 6.5, 30°C Gelatoporia subvermispora
5.6
-
 bicarbonate substrate NAD+, pH 6.5, 30°C Gelatoporia subvermispora