Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.9 extracted from

  • Jiang, W.; Lin, P.; Yang, R.; Fang, B.
    Identification of catalysis, substrate, and coenzyme binding sites and improvement catalytic efficiency of formate dehydrogenase from Candida boidinii (2016), Appl. Microbiol. Biotechnol., 100, 8425-8437.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells [Candida] boidinii

Protein Variants

Protein Variants Comment Organism
N187D the mutant shows increased catalytic efficiency compared to the wild type enzyme [Candida] boidinii
V120S the mutant has the highest catalytic efficiency with formate as it enhances the kcat and kcat/Km values 3.48fold and 1.6fold, respectively, compared to the wild type enzyme [Candida] boidinii
V120S/N187D the mutant has the highest catalytic efficiency with NAD+ as it displays an approximately 1.5fold increase in kcat/Km compared to the wild type enzyme [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.02
-
 NAD+ mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii
2.5
-
 formate mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii
3.4
-
 NAD+ wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
3.6
-
 formate wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
3.8
-
 NAD+ mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
6.7
-
 formate mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
7.9
-
 formate mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii
8.8
-
 NAD+ mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ [Candida] boidinii ATCC 32195
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii O13437
-
-
[Candida] boidinii ATCC 32195 O13437
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?
formate + NAD+
-
 [Candida] boidinii ATCC 32195 CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 the enzyme remains stable after 1 h at 30-40°C [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 formate mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii
1.53
-
 formate wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
3.29
-
 formate mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
5.34
-
 formate mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii
18.68
-
 NAD+ mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii
22.3
-
 NAD+ wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
28.99
-
 NAD+ mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
40.63
-
 NAD+ mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.275
-
 formate mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii
0.423
-
 formate wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
0.491
-
 formate mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
0.676
-
 formate mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii
5.008
-
 NAD+ mutant enzyme V120S, at pH 9.5 and 30°C [Candida] boidinii
6.618
-
 NAD+ wild type enzyme, at pH 9.5 and 30°C [Candida] boidinii
9.808
-
 NAD+ mutant enzyme N187D, at pH 9.5 and 30°C [Candida] boidinii
9.934
-
 NAD+ mutant enzyme V120S/N187D, at pH 9.5 and 30°C [Candida] boidinii