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Literature summary for 1.17.1.9 extracted from

  • Alekseeva, A.A.; Fedorchuk, V.V.; Zarubina, S.A.; Sadykhov, E.G.; Matorin, A.D.; Savin, S.S.; Tishkov, V.I.
    The role of ala198 in the stability and coenzyme specificity of bacterial formate dehydrogenases (2015), Acta Naturae, 7, 60-69 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Moraxella sp. C-1
expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. 101

Protein Variants

Protein Variants Comment Organism
A198G the mutant shows 1.8 fold increased activity compared to the wild type enzyme Moraxella sp. C-1
A198G the mutation reduces the KM value for NAD+ while the KM for CO2 remains practically unchanged, compared to the wild type enzyme Pseudomonas sp. 101
A198G/D221S the mutant has its coenzyme specificity changed from NAD+ to NADP+ compared to the wild type enzyme Pseudomonas sp. 101
D221S the mutant has its coenzyme specificity changed from NAD+ to NADP+ compared to the wild type enzyme Pseudomonas sp. 101

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Pseudomonas sp. 101
0.045
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Moraxella sp. C-1
0.06
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Pseudomonas sp. 101
0.08
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Moraxella sp. C-1
0.19
-
 NADP+ mutant enzyme D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
0.54
-
 NAD+ mutant enzyme A198G/D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
0.71
-
 NAD+ mutant enzyme D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
6.5
-
 CO2 wild type enzyme, pH and temperature not specified in the publication Pseudomonas sp. 101
7.5
-
 CO2 mutant enzyme A198G, pH and temperature not specified in the publication Pseudomonas sp. 101
7.7
-
 CO2 wild type enzyme, pH and temperature not specified in the publication Moraxella sp. C-1
8
-
 CO2 mutant enzyme A198G, pH and temperature not specified in the publication Moraxella sp. C-1
32
-
 CO2 mutant enzyme D221S, pH and temperature not specified in the publication Pseudomonas sp. 101

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Moraxella sp. C-1
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ Pseudomonas sp. 101
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Moraxella sp. C-1 O08375
-
-
Pseudomonas sp. 101 P33160
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and Sephadex G-25 gel filtration Moraxella sp. C-1
phenyl Sepharose column chromatography and Sephadex G-25 gel filtration Pseudomonas sp. 101

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Moraxella sp. C-1 CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Pseudomonas sp. 101 CO2 + NADH + H+
-
 ?
formate + NADP+ NADP+ is a substrate for mutant enzyme D221S only Pseudomonas sp. 101 CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Moraxella sp. C-1
FDH
-
 Pseudomonas sp. 101

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 NAD+ mutant enzyme D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
7.3
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Moraxella sp. C-1
7.3
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Pseudomonas sp. 101
7.3
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Moraxella sp. C-1
7.3
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Pseudomonas sp. 101

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Moraxella sp. C-1
NAD+
-
 Pseudomonas sp. 101

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.04
-
 NAD+ mutant enzyme D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
9.26
-
 NAD+ mutant enzyme A198G/D221S, pH and temperature not specified in the publication Pseudomonas sp. 101
91.3
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Moraxella sp. C-1
122
-
 NAD+ wild type enzyme, pH and temperature not specified in the publication Pseudomonas sp. 101
162
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Moraxella sp. C-1
209
-
 NAD+ mutant enzyme A198G, pH and temperature not specified in the publication Pseudomonas sp. 101