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Literature summary for 1.17.3.2 extracted from

  • Della Corte, E.; Stirpe, F.
    The regulation of rat liver xanthine oxidase. Involvement of thiol groups in the conversion of the enzyme activity from dehydrogenase (type D) into oxidase (type O) and purification of the enzyme (1972), Biochem. J., 126, 739-745.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
the enzyme from animal tissues can be interconverted to EC 1.1.1.204, that from liver exists in vivo mainly as the dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-molybdenum protein Rattus norvegicus
Molybdenum an iron-molybdenum protein Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme from animal tissues can be interconverted to xanthine dehydrogenase, EC 1.1.1.204, the liver enzyme exists in vivo mainly in its dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Rattus norvegicus
proteolytic modification
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, hydroxyapatite, DEAE-cellulose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
189.6
-
electron acceptor O2 Rattus norvegicus
800
-
electron acceptors O2 and NAD+ Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + 2 H2O + 2 O2
-
Rattus norvegicus urate + 2 H2O2
-
?
xanthine + H2O + O2 electron acceptor O2 Rattus norvegicus uric acid + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Rattus norvegicus