Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-6-hydroxy-8-mercaptopurine | mixed-type inhibition, the inhibitor specifically blocks the enzyme activity with the drug 6-mercaptopurine, but does affect activity with xanthine and hypoxanthine to a lesser extent, overview | Bos taurus | |
2-amino-6-purine thiol | competitive inhibitor, the inhibitor specifically blocks the enzyme activity with the drug 6-mercaptopurine, but does affect activity with xanthine and hypoxanthine to a lesser extent, overview | Bos taurus | |
allopurinol | a suicide inhibitor of XOD, a pyrazolopyrimidine derivative, and an analogue of hypoxanthine | Bos taurus | |
additional information | substrate-dependent inhibitory potencies, overview | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-mercaptopurine + H2O + O2 | Bos taurus | an anticancer drug | ? | - |
? | |
hypoxanthine + 2 H2O + 2 O2 | Bos taurus | - |
urate + 2 H2O2 | - |
? | |
xanthine + H2O + O2 | Bos taurus | from dead cancer cells | urate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-mercaptopurine + 2 H2O + 2 O2 | production of superoxide radicals | Bos taurus | 6-thiouric acid + 2 H2O2 | - |
? | |
6-mercaptopurine + H2O + O2 | an anticancer drug | Bos taurus | ? | - |
? | |
hypoxanthine + 2 H2O + 2 O2 | - |
Bos taurus | urate + 2 H2O2 | - |
? | |
hypoxanthine + 2 H2O + 2 O2 | via intermediate xanthine formation, production of superoxide radicals | Bos taurus | urate + 2 H2O2 | - |
? | |
xanthine + H2O + O2 | from dead cancer cells | Bos taurus | urate + H2O2 | - |
? | |
xanthine + H2O + O2 | production of superoxide radicals | Bos taurus | urate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XOD | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bos taurus | |
0.00096 | - |
2-amino-6-hydroxy-8-mercaptopurine | pH 7.4, competitive versus substrate 6-mercaptopurine | Bos taurus | |
0.00098 | - |
2-amino-6-hydroxy-8-mercaptopurine | pH 7.4, noncompetitive versus substrate 6-mercaptopurine | Bos taurus | |
0.0013 | - |
2-amino-6-purine thiol | pH 7.4, competitive versus substrate 6-mercaptopurine | Bos taurus | |
0.0057 | - |
2-amino-6-hydroxy-8-mercaptopurine | pH 7.4, competitive versus substrate xanthine | Bos taurus | |
0.00624 | - |
2-amino-6-hydroxy-8-mercaptopurine | pH 7.4, noncompetitive versus substrate xanthine | Bos taurus | |
0.0066 | - |
2-amino-6-purine thiol | pH 7.4, competitive versus substrate xanthine | Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
in vitro bi-substrate-inhibitor-enzyme simulation and kinetics, overview | Bos taurus | additional information | |
0.00054 | - |
pH 7.4, versus substrate 6-mercaptopurine | Bos taurus | 2-amino-6-hydroxy-8-mercaptopurine | |
0.00091 | - |
pH 7.4, versus substrate hypoxanthine | Bos taurus | allopurinol | |
0.00192 | - |
pH 7.4, versus substrate 6-mercaptopurine | Bos taurus | allopurinol | |
0.00236 | - |
pH 7.4, versus substrate xanthine | Bos taurus | allopurinol | |
0.0026 | - |
pH 7.4, versus substrate 6-mercaptopurine | Bos taurus | 2-amino-6-purine thiol | |
0.0051 | - |
pH 7.4, versus substrate hypoxanthine | Bos taurus | 2-amino-6-hydroxy-8-mercaptopurine | |
0.0073 | - |
pH 7.4, versus substrate hypoxanthine | Bos taurus | 2-amino-6-purine thiol | |
0.0164 | - |
pH 7.4, versus substrate xanthine | Bos taurus | 2-amino-6-purine thiol | |
0.0177 | - |
pH 7.4, versus substrate xanthine | Bos taurus | 2-amino-6-hydroxy-8-mercaptopurine |