Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.3.2 extracted from

  • Chang, Y.C.; Lee, F.W.; Chen, C.S.; Huang, S.T.; Tsai, S.H.; Huang, S.H.; Lin, C.M.
    Structure-activity relationship of C6-C3 phenylpropanoids on xanthine oxidase-inhibiting and free radical-scavenging activities (2007), Free Radic. Biol. Med., 43, 1541-1551.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-coumaric acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
2-methoxycinnamic acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
3-coumaric acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
3-methoxycinnamic acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
4-coumaric acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
4-methoxycinnamic acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
allopurinol 36.6% inhibition at 0.04 mM Mus musculus
aminoguanidine 78.6% inhibition at 0.04 mM Mus musculus
ascorbate 43.5% inhibition at 0.04 mM Mus musculus
caffeic acid 42.8% inhibition at 0.04 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
caffeic acid phenethyl ester 55.0% inhibition at 0.004 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
Cinnamic acid 40.2% inhibition at 0.04 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
ferulic acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
isoferulic acid competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview Mus musculus
additional information inhibitory effects of phenylpropanoids on DNA relaxation activities, 1,1-diphenyl-2-picrylhydrazyl, and 5,5-dimethyl-1-pyrroline-N-oxide, and on iron-induced hydroxyl radical formation, hydroxyl radical-scavenger properties of the compounds, overview, 3D modelling of docking Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + H2O + O2 Mus musculus production of superoxide radicals, treatment with an enzyme inhibitor largely prevents the development of endothelial dysfunction and atherosclerosis in mice urate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
RAW-264.7 cell a mouse monocyte-macrophage cell line, ATCC TIB-71 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthine + H2O + O2 production of superoxide radicals Mus musculus urate + H2O2
-
?
xanthine + H2O + O2 production of superoxide radicals, treatment with an enzyme inhibitor largely prevents the development of endothelial dysfunction and atherosclerosis in mice Mus musculus urate + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00143
-
pH 8.0, 37°C, competitive versus xanthine Mus musculus allopurinol
0.00626
-
pH 8.0, 37°C, competitive versus xanthine Mus musculus caffeic acid phenethyl ester
0.0654
-
pH 8.0, 37°C, competitive versus xanthine Mus musculus caffeic acid
0.0654
-
pH 8.0, 37°C, versus xanthine Mus musculus caffeic acid
0.0939
-
pH 8.0, 37°C, versus xanthine Mus musculus ferulic acid
0.0969
-
pH 8.0, 37°C, versus xanthine Mus musculus 4-coumaric acid
0.1432
-
pH 8.0, 37°C, versus xanthine Mus musculus isoferulic acid
0.1567
-
pH 8.0, 37°C, inhibition of hydroxylradical formation in the dimethyl-1-pyrroline-N-oxide spin-trapping assay Mus musculus caffeic acid phenethyl ester
0.184
-
pH 8.0, 37°C, versus xanthine Mus musculus 4-methoxycinnamic acid