KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
CDP | subunit L1 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 120000 Da L2 subunit of regenerating liver contains iron | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |
45000 | - |
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE | Rattus norvegicus |
75000 | - |
4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |
75000 | - |
x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta structure, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
regenerating liver, dATP-Sepharose affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | regenerating liver | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0131 | - |
purified subunit L2 assayed in the presenc of L1 | Rattus norvegicus |
0.015 | - |
purified subunit L1 assayed in the presenc of L2, specific for CDP | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 0.25 M sucrose, 100 mM Tris-HCl, pH 7.6, 10 mM MgCl2, 2 mM dithiothreitol, 1 month, no loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Rattus norvegicus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000 + 1 * 75000 + 1 * 45000, holoenzyme may have an alpha4,beta,beta' structure, SDS-PAGE | Rattus norvegicus |
hexamer | 4 * 45000 + 1 * 45000 + 1 * 75000, regenerating liver | Rattus norvegicus |