Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.4.1 extracted from

  • Slabaugh, M.B.; Davis, R.E.; Roseman, N.A.; Mathews, C.K.
    Vaccinia virus ribonucleotide reductase expression and isolation of the recombinant large subunit (1993), J. Biol. Chem., 268, 17803-17810.
    View publication on PubMed
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
adenyl-5'-yl-imidodiphosphate stimulation at low concentration, inhibition above 0.3 mM Vaccinia virus
ATP most effective activator Vaccinia virus
P1,P4-bis(5'-adenosyl) tetraphosphate stimulation at low concentrations, inhibition above 0.3 mM Vaccinia virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vaccinia virus

General Stability

General Stability Organism
quite susceptible to denaturation Vaccinia virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2fold activation Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Blue Sepharose, affinity resin Vaccinia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.122
-
 recombinant enzyme Vaccinia virus

Storage Stability

Storage Stability Organism
-80°C, several months, no loss of activity Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM Vaccinia virus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.8
-
 Vaccinia virus

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.8 60% activity at pH 7.2 Vaccinia virus