Literature summary for 1.17.4.1 extracted from

Ogata, H.; Stolle, P.; Stehr, M.; Auling, G.; Lubitz, W.
Crystallization and preliminary X-ray analysis of the small subunit (R2F) of native ribonucleotide reductase from Corynebacterium ammoniagenes (2009), Acta Crystallogr. Sect. F, 65, 878-880.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of R2F using expression vector pOCA2	Corynebacterium ammoniagenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
R2F, sitting drop vapour diffusion method, at 4°C, mixing 0.001 ml of RNR solution, containing mM KCl, 50 mM TrisHCl, 2 mM DTT, 15% glycerol, pH 7.5, with 0.001 ml of reservoir buffer solution containing 0.1 M sodium citrate, 27.5% PEG 4000, 0.05 M ammonium acetate, pH 6.0, and 0.1 M ammonium acetate pH 7.0 with 0.05 MTris-HCl, pH 7.5, X-ray diffraction structure determination and analysis at 1.36 A resolution	Corynebacterium ammoniagenes

Crystallization (Comment)

Organism

R2F, sitting drop vapour diffusion method, at 4°C, mixing 0.001 ml of RNR solution, containing mM KCl, 50 mM TrisHCl, 2 mM DTT, 15% glycerol, pH 7.5, with 0.001 ml of reservoir buffer solution containing 0.1 M sodium citrate, 27.5% PEG 4000, 0.05 M ammonium acetate, pH 6.0, and 0.1 M ammonium acetate pH 7.0 with 0.05 MTris-HCl, pH 7.5, X-ray diffraction structure determination and analysis at 1.36 A resolution

Corynebacterium ammoniagenes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	R2F is a mangagnese-containing enzyme	Corynebacterium ammoniagenes
additional information	R2F does not contain the metals Fe, Co, Ni and Cu	Corynebacterium ammoniagenes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Corynebacterium ammoniagenes	ribonucleotide reduction is the unique step in DNA-precursor biosynthesis and involves radical-dependent redox chemistry and diverse metallo-cofactors, overview. The Mn-RNR from the Gram-positive bacterium Corynebacterium ammoniagenes, strain ATCC 6872, belongs a distinct RNR class IV enzyme	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium ammoniagenes	-	strain ATCC 6872, gene nrdF	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant R2F by anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography to homogeneity	Corynebacterium ammoniagenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	ribonucleotide reduction is the unique step in DNA-precursor biosynthesis and involves radical-dependent redox chemistry and diverse metallo-cofactors, overview. The Mn-RNR from the Gram-positive bacterium Corynebacterium ammoniagenes, strain ATCC 6872, belongs a distinct RNR class IV enzyme	Corynebacterium ammoniagenes	?	-	?

Synonyms

Synonyms	Comment	Organism
Mn-RNR	-	Corynebacterium ammoniagenes
R2F	-	Corynebacterium ammoniagenes
ribonucleotide reductase	-	Corynebacterium ammoniagenes