Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | activates | Bacillus subtilis | |
dATP | activates | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally tagged NrdE and NrdF, the RNR genes are organized within the nrdI-nrdE-nrdF-ymaB operon, recombinant expression of NrdI, NdE, NrdF, and YmaB in Escherichia coli. For overexpression, the entire operon is placed behind a Pspank(hy) promoter and integrated into the Bacillus subtilis genome at the amyE site. Removal of the tag from rNrdE and rNrdF, leaving three amino acids GSH, has little effect on activity | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the isolated recombinant NrdF contains a diferric-tyrosyl radical [Fe(III)2-Y.] cofactor | Bacillus subtilis | |
Mg2+ | class Ib ribonucleotide reductase is a dimanganese(III)-tyrosyl radical enzyme, with Tyr115. Subunit beta, NrdF, contains the metallo-cofactor, essential for the initiation of the reduction process | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis JH624 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant NrdI, NdE, NrdF, and YmaB from Escherichia coli. The absorption spectra of the purified NrdENrdF complex exhibited characteristics of a Mn(III)2-Y radical center with 2 Mn/beta2 and 0.5 Y radical/beta2 | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCDP + DTT disulfide + H2O | in the assays for the Fe- and Mn-loaded recombinant NrdF, a 10fold excess of recombinant NrdE is used, CDP is the substrate, ATP or dATP is the effector, and DTT is the reductant | Bacillus subtilis | CDP + DTT | - |
r | |
dCDP + DTT disulfide + H2O | in the assays for the Fe- and Mn-loaded recombinant NrdF, a 10fold excess of recombinant NrdE is used, CDP is the substrate, ATP or dATP is the effector, and DTT is the reductant | Bacillus subtilis JH624 | CDP + DTT | - |
r | |
additional information | in the presence of rNrdE, ATP, and CDP, Mn(III)2-Y* and Fe(III)2-Y* rNrdF generate dCDP at rates of 132 and 10 nmol min/mg, respectively | Bacillus subtilis | ? | - |
? | |
additional information | in the presence of rNrdE, ATP, and CDP, Mn(III)2-Y* and Fe(III)2-Y* rNrdF generate dCDP at rates of 132 and 10 nmol min/mg, respectively | Bacillus subtilis JH624 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alphabeta, class Ib RNR is composed of two subunits alpha (NrdE) and beta (NrdF). Beta contains the metallo-cofactor, essential for the initiation of the reduction process | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
class Ib ribonucleotide reductase | - |
Bacillus subtilis |
class Ib RNR | - |
Bacillus subtilis |
NrdE | - |
Bacillus subtilis |
NrdF | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | RNR genes are induced in cells grown in Luria-Bertani medium, with levels of NrdE and NrdF elevated 35fold relative to that of the wild-type strain | up |
General Information | Comment | Organism |
---|---|---|
physiological function | RNRs catalyze the conversion of nucleotides UDP, ADP, GDP, and CDP to deoxynucleotides, providing the monomeric building blocks required for DNA replication and repair | Bacillus subtilis |