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Literature summary for 1.17.4.2 extracted from

  • Sando, G.N.; Hogenkamp, H.P.C.
    Ribonucleotide reductase from Thermus X-1, a thermophilic organism (1973), Biochemistry, 12, 3316-3322.
    View publication on PubMed
Search for more literature for 1.17.4.2

Activating Compound

Activating Compound Comment Organism Structure
5'-deoxy-5'-adenosylcobalamin
-
 Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 GTP at 37°C Thermus sp.
0.32
-
 CTP at 37°C Thermus sp.
0.99
-
 GTP at 70°C Thermus sp.
1
-
 ATP at 70°C Thermus sp.
1.4
-
 CTP at 70°C Thermus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates enzyme activity Thermus sp.
Mn2+ stimulates enzyme activity Thermus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus sp.
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O Thermus aquaticus YT-1
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
-
-
Thermus aquaticus
-
-
-
Thermus aquaticus YT-1
-
-
-
Thermus sp.
-
 X-1
-
Thermus sp. X-1
-
 X-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactobacillus leichmannii
partially Thermus sp.

Storage Stability

Storage Stability Organism
20°C, 0.03 M dimethylglutarate buffer, pH 7.2, remains fully active on storage for 24 h Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + reduced thioredoxin
-
 Thermus sp. dADP + oxidized thioredoxin + H2O
-
 ?
ADP + reduced thioredoxin
-
 Lactobacillus leichmannii dADP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Thermus sp. dATP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Lactobacillus leichmannii dATP + oxidized thioredoxin + H2O
-
 ?
ATP + reduced thioredoxin
-
 Thermus sp. X-1 dATP + oxidized thioredoxin + H2O
-
 ?
CDP + reduced thioredoxin
-
 Thermus sp. dCDP + oxidized thioredoxin + H2O
-
 ?
CDP + reduced thioredoxin
-
 Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
 ?
CMP + reduced thioredoxin
-
 Thermus sp. dCMP + oxidized thioredoxin + H2O
-
 ?
CMP + reduced thioredoxin
-
 Lactobacillus leichmannii dCMP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Thermus sp. dCTP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
 ?
CTP + reduced thioredoxin
-
 Thermus sp. X-1 dCTP + oxidized thioredoxin + H2O
-
 ?
GDP + reduced thioredoxin
-
 Thermus sp. dGDP + oxidized thioredoxin + H2O
-
 ?
GDP + reduced thioredoxin
-
 Lactobacillus leichmannii dGDP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Thermus sp. dGTP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Lactobacillus leichmannii dGTP + oxidized thioredoxin + H2O
-
 ?
GTP + reduced thioredoxin
-
 Thermus sp. X-1 dGTP + oxidized thioredoxin + H2O
-
 ?
IDP + reduced thioredoxin
-
 Thermus sp. dIDP + oxidized thioredoxin
-
 ?
ITP + reduced thioredoxin
-
 Thermus sp. dITP + oxidized thioredoxin
-
 ?
additional information GMP and AMP are no substrates Thermus sp. ?
-
 ?
additional information GMP and AMP are no substrates Thermus sp. X-1 ?
-
 ?
ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus sp. X-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin
-
 Thermus aquaticus YT-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermus aquaticus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermus sp. 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermus aquaticus YT-1 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
UDP + reduced thioredoxin
-
 Thermus sp. dUDP + oxidized thioredoxin + H2O
-
 ?
UDP + reduced thioredoxin
-
 Lactobacillus leichmannii dUDP + oxidized thioredoxin + H2O
-
 ?
UMP + reduced thioredoxin
-
 Thermus sp. dUMP + oxidized thioredoxin + H2O
-
 ?
UTP + reduced thioredoxin
-
 Thermus sp. dUTP + oxidized thioredoxin + H2O
-
 ?
UTP + reduced thioredoxin
-
 Lactobacillus leichmannii dUTP + oxidized thioredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Lactobacillus leichmannii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lactobacillus leichmannii
70
-
-
 Thermus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
 Lactobacillus leichmannii
20 80
-
 Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.4
-
 Thermus sp.

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 adenosylcobalamin Lactobacillus leichmannii