Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Escherichia coli | |
cyanide | - |
Escherichia coli | |
iodoacetamide | - |
Escherichia coli | |
O2 | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
formate | 30°C, per mol of heme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide | Escherichia coli | |
Mo | the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide | Escherichia coli | |
additional information | contains neither FAD nor FMN | Escherichia coli | |
Se | the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide. The alpha polypeptide contains significant amounts of selenium | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE | Escherichia coli |
32000 | - |
x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE | Escherichia coli |
110000 | - |
x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE | Escherichia coli |
590000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
formate dehydrogenase activity is lost when the enzyme is exposed to oxygen. This instability is enhanced in low ionic strength buffers, detergents, at temperatures above 0°C, and at pH greater than 7 | Escherichia coli |
formate dehydrogenase is extremely sensitive to inactivation by oxygen in the presence of formate. Purified formate dehydrogenase is completely inactivated by aerobic incubation in 75 mM sodium phosphate, pH 7, for 1 h at 20°C in the presence of 50 mM formate, while only 40% of the activity is lost when the same incubation is carried out in the absence of formate. No activity is lost when the same incubation is carried out anaerobically even in the presence of formate | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
196 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2,3-dimethoxy-5-methyl-6-(farnesylfarnesyl)-1,4-benzoquinone | - |
Escherichia coli | CO2 + ? | - |
? | |
formate + 2,6-dichlorophenolindophenol | dichlorophenylindophenol reduction in presence of phenazine methosulfate | Escherichia coli | CO2 + reduced 2,6-dichlorophenolindophenol | - |
ir | |
formate + benzyl viologen | - |
Escherichia coli | CO2 + reduced benzyl viologen | - |
? | |
formate + ferricyanide | - |
Escherichia coli | CO2 + ferrocyanide + H+ | - |
? | |
formate + methyl viologen | - |
Escherichia coli | CO2 + reduced methyl viologen | - |
ir | |
formate + methylene blue | - |
Escherichia coli | CO2 + reduced methylene blue | - |
? | |
formate + nitroblue tetrazolium | - |
Escherichia coli | CO2 + reduced nitroblue tetrazolium | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 8.5, 0.75 M Tris-HCl, anaerobic condition, stable for 15 min | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
563 | - |
formate | 30°C, per mol of heme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide | Escherichia coli |