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Literature summary for 1.17.9.1 extracted from
- Genes, enzymes, and regulation of para-cresol metabolism in Geobacter metallireducens (2007), J. Bacteriol., 189, 4729-4738.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | a tightly membrane-bound PCMH complex exists in anaerobic organisms in cotrast to aerobic ones, where the enzyme is soluble and periplasmic, overview | Geobacter metallireducens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a cytochrome c-containing enzyme | Geobacter metallireducens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | Geobacter metallireducens | the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction | 4-hydroxybenzyl alcohol + reduced acceptor | - |
? | |
| additional information | Geobacter metallireducens | pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacter metallireducens | - |
two isozymes encoded by genes pcmI and pcmJ | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
cell extract, substrate 4-hydroxybenzyl alcohol | Geobacter metallireducens |
| 0.025 | - |
cell extract, substrate 4-cresol | Geobacter metallireducens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-cresol + acceptor + H2O | - |
Geobacter metallireducens | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
| 4-cresol + acceptor + H2O | the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction | Geobacter metallireducens | 4-hydroxybenzyl alcohol + reduced acceptor | - |
? | |
| 4-cresol + ferricenium + H2O | PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors | Geobacter metallireducens | 4-hydroxybenzyl alcohol + ferrocenium | - |
? | |
| 4-cresol + NAD(P)+ + H2O | PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors | Geobacter metallireducens | 4-hydroxybenzyl alcohol + NAD(P)H | - |
? | |
| 4-cresol + phenazine methosulfate + H2O | PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors | Geobacter metallireducens | 4-hydroxybenzyl alcohol + reduced phenazine methosulfate | - |
? | |
| additional information | pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview | Geobacter metallireducens | ? | - |
? | |
| additional information | an unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of 4-hydroxybenzyl alcohol oxidation, overview, no activity with 4-hydroxybenzoate, benzoate, and acetate | Geobacter metallireducens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of p-hydroxybenzyl alcohol oxidation, overview | Geobacter metallireducens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| p-cresol methylhydroxylase | - |
Geobacter metallireducens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Geobacter metallireducens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Geobacter metallireducens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | active in 4-cresol oxidation | Geobacter metallireducens | |
| FAD | - |
Geobacter metallireducens | |
| menaquinone | active in 4-hydroxybenzyl alcohol oxidation | Geobacter metallireducens | |
| NAD+ | - |
Geobacter metallireducens | |
| NADP+ | - |
Geobacter metallireducens | |
| phenazine methosulfate | - |
Geobacter metallireducens | |
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