KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
formate | pH 7.5, temperature not specified in the publication, substrate: deuterioformate | Methanobacterium formicicum | |
0.012 | - |
formate | pH 7.5, temperature not specified in the publication, substrate: protioformate | Methanobacterium formicicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + oxidized coenzyme F420 | Methanobacterium formicicum | - |
CO2 + reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium formicicum | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formate + oxidized coenzyme F420 = CO2 + reduced coenzyme F420 | the enzyme is specific for the si face hydride transfer to C5 of coenzyme F420. While catalysis probably occurs by hydride transfer from formate to the enzyme to generate an enzyme-H2 species and then by hydride transfer back out to F420, the formate-derived hydrogen exchanges with solvent protons before transfer back out to coenzyme F420. The kinetics of hydride transfer from formate reveals that this step is not rate determining | Methanobacterium formicicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + oxidized coenzyme F420 | - |
Methanobacterium formicicum | CO2 + reduced coenzyme F420 | - |
? | |
formate + oxidized methyl viologen | the enzyme is specific for the si face hydride transfer to C5 of coenzyme F420 | Methanobacterium formicicum | CO2 + reduced methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
coenzyme F420 reducing formate dehydrogenase | - |
Methanobacterium formicicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanobacterium formicicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group, Km: 0.013 mM | Methanobacterium formicicum |