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Literature summary for 1.17.98.3 extracted from

  • Schauer, N.L.; Ferry, J.G.; Honek, J.F.; Orme-Johnson, W.H.; Walsh, C.
    Mechanistic studies of the coenzyme F420 reducing formate dehydrogenase from Methanobacterium formicicum (1986), Biochemistry, 25, :7163-7168.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 formate pH 7.5, temperature not specified in the publication, substrate: deuterioformate Methanobacterium formicicum
0.012
-
 formate pH 7.5, temperature not specified in the publication, substrate: protioformate Methanobacterium formicicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + oxidized coenzyme F420 Methanobacterium formicicum
-
 CO2 + reduced coenzyme F420
-
 ?

Organism

Organism UniProt Comment Textmining
Methanobacterium formicicum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
formate + oxidized coenzyme F420 = CO2 + reduced coenzyme F420 the enzyme is specific for the si face hydride transfer to C5 of coenzyme F420. While catalysis probably occurs by hydride transfer from formate to the enzyme to generate an enzyme-H2 species and then by hydride transfer back out to F420, the formate-derived hydrogen exchanges with solvent protons before transfer back out to coenzyme F420. The kinetics of hydride transfer from formate reveals that this step is not rate determining Methanobacterium formicicum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + oxidized coenzyme F420
-
 Methanobacterium formicicum CO2 + reduced coenzyme F420
-
 ?
formate + oxidized methyl viologen the enzyme is specific for the si face hydride transfer to C5 of coenzyme F420 Methanobacterium formicicum CO2 + reduced methyl viologen
-
 ?

Synonyms

Synonyms Comment Organism
coenzyme F420 reducing formate dehydrogenase
-
 Methanobacterium formicicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methanobacterium formicicum

Cofactor

Cofactor Comment Organism Structure
FAD prosthetic group, Km: 0.013 mM Methanobacterium formicicum