Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mo2+ | molybdoenzyme | Sterolibacterium denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + acceptor + H2O | Sterolibacterium denitrificans | - |
25-hydroxycholest-4-en-3-one + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sterolibacterium denitrificans | H9NN89 AND H9NN90 AND H9NN91 | H9NN89: steroid C25 dehydrogenase alpha subunit, H9NN90: steroid C25 dehydrogenase beta subunit, H9NN91: steroid C25 dehydrogenase gamma subunit | - |
Oxidation Stability | Organism |
---|---|
purified enzyme is inactivated irreversibly by incubation in air with a half-life of 14 min. The inactivation is largely prevented by the addition of the artificial electron acceptor potassium hexacyanoferrate (III) to the enzyme preparation. In the presence of cyanoferrate, more than 90% of the enzyme activity is retained after a 2 h incubation in air. The enzymatic activity is completely lost after 24 h of incubation | Sterolibacterium denitrificans |
Storage Stability | Organism |
---|---|
the enzyme can be stored for at least 3 months at 4 °C under anaerobic conditions | Sterolibacterium denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-cholesten-3beta-ol-7-one + acceptor + H2O | 15% of the activity as compared to activity with cholest-4-en-3-one | Sterolibacterium denitrificans | 25-hydroxy-5-cholesten-3beta-ol-7-one + reduced acceptor | - |
? | |
cholest-4-en-3-one + acceptor + H2O | - |
Sterolibacterium denitrificans | 25-hydroxycholest-4-en-3-one + reduced acceptor | - |
? | |
cholesta-4,6-dien-3-one + acceptor + H2O | 127% of the activity as compared to activity with cholest-4-en-3-one | Sterolibacterium denitrificans | 25-hydroxycholesta-4,6-dien-3-one + reduced acceptor | - |
? | |
cholesterol + acceptor + H2O | 10% of the activity as compared to activity with cholest-4-en-3-one | Sterolibacterium denitrificans | 25-hydroxycholesterol + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C25 dehydrogenase | - |
Sterolibacterium denitrificans |