Cloned (Comment) | Organism |
---|---|
expressed in Bacillus megaterium DSM319 cells | Streptococcus thermophilus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.3 M sodium acetate, 0.1 M Tris-HCl, pH 7.5, 15% (w/v) PEG 4000 | Streptococcus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
queuosine34 in tRNA + acceptor + H2O | Streptococcus thermophilus | - |
epoxyqueuosine34 in tRNA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus thermophilus | A0A0R4I997 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and heparin column chromatography | Streptococcus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxyqueuosine34 in tRNA + reduced methyl viologen | - |
Streptococcus thermophilus | queuosine34 in tRNA + methyl viologen + H2O | - |
? | |
queuosine34 in tRNA + acceptor + H2O | - |
Streptococcus thermophilus | epoxyqueuosine34 in tRNA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Streptococcus thermophilus |
? | x * 47019, electrospray ionization mass spectrometry | Streptococcus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
QueG | - |
Streptococcus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | - |
Streptococcus thermophilus | |
[4Fe-4S]-center | - |
Streptococcus thermophilus |