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Literature summary for 1.18.1.1 extracted from

  • Perry, A.; Tambyrajah, W.; Grossmann, J.G.; Lian, L.Y.; Scrutton, N.S.
    Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by NMR spectroscopy and solution X-ray scattering and interactions with rubredoxin reductase (2004), Biochemistry, 43, 3167-3182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TG1 Pseudomonas oleovorans

General Stability

General Stability Organism
20% glycerol protects against inactivation, prevents loss of FAD from the intact enzyme Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced rubredoxin + NAD+ Pseudomonas oleovorans
-
oxidized rubredoxin + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced rubredoxin + NAD+
-
Pseudomonas oleovorans oxidized rubredoxin + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas oleovorans
NAD+
-
Pseudomonas oleovorans