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Literature summary for 1.18.1.2 extracted from
- The oxidant-responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)-NADP(H) reductase (2003), FEBS Lett., 553, 408-412.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fpr, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Rhodobacter capsulatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
about, gel filtration | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced ferredoxin + NADP+ | Rhodobacter capsulatus | - |
oxidized ferredoxin + NADPH | - |
? | |
| reduced ferredoxin + NADP+ | Rhodobacter capsulatus 37b4 | - |
oxidized ferredoxin + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | Q9L6V3 | - |
- |
| Rhodobacter capsulatus 37b4 | Q9L6V3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli to homogeneity, native enzyme about 400fold to homogeneity | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus | reduced methyl viologen + NADP+ | - |
? | |
| methyl viologen + NADPH | diaphorase activity | Rhodobacter capsulatus 37b4 | reduced methyl viologen + NADP+ | - |
? | |
| reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized ferredoxin + NADPH | - |
? | |
| reduced ferredoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized ferredoxin + NADPH | - |
? | |
| reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus | oxidized flavodoxin + NADPH + H+ | - |
r | |
| reduced flavodoxin + NADP+ | - |
Rhodobacter capsulatus 37b4 | oxidized flavodoxin + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, about, recombinant and native enzyme, SDS-PAGE | Rhodobacter capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase | - |
Rhodobacter capsulatus |
| ferredoxin (flavodoxin)-NADP(H) reductase | - |
Rhodobacter capsulatus |
| FPR | - |
Rhodobacter capsulatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
reduced flavodoxin | pH 8.0 | Rhodobacter capsulatus | |
| 7.2 | - |
NADPH | diaphorase activity, pH 8.0 | Rhodobacter capsulatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | prosthetic group, tightly bound, can be released by heat denaturation | Rhodobacter capsulatus | |
| NADP+ | - |
Rhodobacter capsulatus | |
| NADPH | - |
Rhodobacter capsulatus | |
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Release 2023.1 (January 2023)
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