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Literature summary for 1.18.1.2 extracted from
- Ferredoxin-NADP+ reductase from Pseudomonas putida functions as a ferric reductase (2009), J. Bacteriol., 191, 1472-1479.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Fprs are induced by Fe2+ depletion but not by Fe2+ excess | Pseudomonas putida |  |
| Fe3+ | 0.5 microM Fe(III)-citrate and 0.5 microM Fe(III)-EDTA induces Fprs | Pseudomonas putida | |
| H2O2 | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
| menadione | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
| paraquat | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
| tert-butyl hydroperoxide | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00235 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00273 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00338 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00457 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.00459 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00587 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00616 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00625 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.00636 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.0065 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.00666 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.007 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.00751 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.00866 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.01177 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.01238 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.0129 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.01319 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 0.01424 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.01544 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.01866 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.02967 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.05625 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 0.1076 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
wild type and mutants | - |
| Pseudomonas putida KT 2240 | - |
wild type and mutants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.05 | - |
electron donor NADH, cofactor FAD | Pseudomonas putida |
| 2.58 | - |
electron donor NADPH, cofactor FAD | Pseudomonas putida |
| 5.47 | - |
electron donor NADH, cofactor FMN | Pseudomonas putida |
| 5.73 | - |
electron donor NADPH, cofactor FMN | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fe(III) citrate + NADH | - |
Pseudomonas putida | Fe(II) citrate + NAD+ | - |
? | |
| Fe(III) citrate + NADH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NAD+ | - |
? | |
| Fe(III) citrate + NADPH | - |
Pseudomonas putida | Fe(II) citrate + NADP+ | - |
? | |
| Fe(III) citrate + NADPH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NADP+ | - |
? | |
| Fe(III)-EDTA + NADH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NAD+ | - |
? | |
| Fe(III)-EDTA + NADH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NAD+ | - |
? | |
| Fe(III)-EDTA + NADPH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NADP+ | - |
? | |
| Fe(III)-EDTA + NADPH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NADP+ | - |
? | |
| NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida | NAD+ + reduced FAD | - |
? | |
| NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida KT 2240 | NAD+ + reduced FAD | - |
? | |
| NADPH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | NADP+ + reduced FAD | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferredoxin-NADP+ reductase | - |
Pseudomonas putida |
| FprA | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
| FprB | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
| Fprs | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.91 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 3.11 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 5.6 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 6.18 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 6.8 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 7.13 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 7.54 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 7.71 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 8.21 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 9.18 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 10 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 10.18 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 10.28 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 10.64 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 10.77 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 11.18 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 11.92 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 13.2 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 16.44 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 17.47 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 19.24 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 20.38 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 21.72 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 37.61 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | free FAD serves as an electron carrier | Pseudomonas putida | |
| FMN | free FMN serves as an electron carrier | Pseudomonas putida | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | function in ferric iron metabolism | Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 90 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 110 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 550 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 720 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 770 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 840 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 930 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 930 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 1060 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 1060 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 1170 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 1190 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 1320 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 1570 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 1680 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 2090 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 2690 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
| 2870 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 3130 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 3270 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 6040 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 7090 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
| 17000 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
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