Literature summary for 1.18.1.2 extracted from

Tondo, M.L.; Musumeci, M.A.; Delprato, M.L.; Ceccarelli, E.A.; Orellano, E.G.
Structural-functional characterization and physiological significance of ferredoxin-NADP reductase from Xanthomonas axonopodis pv. citri (2011), PLoS ONE, 6, e27124.

Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in an enzyme knock-out Eschwerichia coli strain	Xanthomonas axonopodis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0108	-	NADPH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis
3.3	-	NADH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 reduced ferredoxin + NADP+	Xanthomonas axonopodis	ferredoxin Xac1762 is a potential substrate	2 oxidized ferredoxin + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas axonopodis	Q8PMH0	pv. citri	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 reduced ferredoxin + NAD+	NAD+ is a poor substrate	Xanthomonas axonopodis	2 oxidized ferredoxin + NADH + H+	-	?
2 reduced ferredoxin + NADP+	ferredoxin Xac1762 is a potential substrate	Xanthomonas axonopodis	2 oxidized ferredoxin + NADPH + H+	-	?
additional information	no substrates: pea ferredoxin, Escherichia coli ferredoxin, flavodoxin	Xanthomonas axonopodis	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48.1	-	melting temperature	Xanthomonas axonopodis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2	-	NADH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis
121.9	-	NADPH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis

Cofactor

Cofactor	Comment	Organism	Structure
NADH	specificity NADPH/NADH is 1600	Xanthomonas axonopodis
NADPH	specificity NADPH/NADH is 1600	Xanthomonas axonopodis

Expression

Organism	Comment	Expression
Xanthomonas axonopodis	a 2.2fold induction of expression is observed in the presence of the superoxide-generating agents methyl viologen and 2,3-dimethoxy-1,4-naphthoquinone	up

General Information

General Information	Comment	Organism
physiological function	enzyme is able to substitute for the ferredoxin-NADP+-reductase in Escherichia coli in its antioxidant role. It is involved in the oxidative stress response of Xanthomonas axonopodis pv. citri and performs its biological function most likely through the interaction with ferredoxin XAC1762	Xanthomonas axonopodis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1	-	NADH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis
11300	-	NADPH	pH 8.0, temperature not specified in the publication	Xanthomonas axonopodis

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Release 2023.1 (January 2023)