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Literature summary for 1.18.1.3 extracted from

  • Hess, V.; Schuchmann, K.; Müller, V.
    The ferredoxin: NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium woodii requires Na+ and is reversibly coupled to the membrane potential (2013), J. Biol. Chem., 288, 31496-31502.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.1.3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Acetobacterium woodii 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + NAD+ Acetobacterium woodii ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
 r
reduced ferredoxin + NAD+ Acetobacterium woodii DSM 103 ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
 r
reduced ferredoxin + NAD+ Acetobacterium woodii DSM 1030 ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
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-
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Acetobacterium woodii DSM 103
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50000
-
 substrate CO-reduced ferredoxin, pH 7.7, 30°C Acetobacterium woodii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii reduced ferredoxin + NAD+
-
 r
oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 103 reduced ferredoxin + NAD+
-
 r
oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 1030 reduced ferredoxin + NAD+
-
 r
reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii oxidized ferredoxin + NADH + H+
-
 r
reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 103 oxidized ferredoxin + NADH + H+
-
 r
reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 1030 oxidized ferredoxin + NADH + H+
-
 r