Literature summary for 1.18.6.1 extracted from

Winter, H.C.; Burris, R.H.
Nitrogenase (1976), Annu. Rev. Biochem., 45, 409-426.

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Azotobacter vinelandii

Inhibitors

Inhibitors	Comment	Organism
H2	-	Allochromatium vinosum
H2	-	Anabaena cylindrica
H2	-	Azotobacter chroococcum
H2	-	Azotobacter vinelandii
H2	-	Bradyrhizobium japonicum
H2	-	Bradyrhizobium lupini
H2	-	Clostridium pasteurianum
H2	-	Corynebacterium flavescens
H2	-	Klebsiella pneumoniae
H2	-	Paenibacillus polymyxa
H2	-	Rhodospirillum rubrum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	-	Klebsiella pneumoniae
additional information	-	additional information	-	Clostridium pasteurianum
additional information	-	additional information	-	Azotobacter vinelandii
additional information	-	additional information	-	Allochromatium vinosum
additional information	-	additional information	-	Anabaena cylindrica
additional information	-	additional information	-	Azotobacter chroococcum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Bradyrhizobium japonicum	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Klebsiella pneumoniae	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Clostridium pasteurianum	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Azotobacter vinelandii	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Paenibacillus polymyxa	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Rhodospirillum rubrum	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Allochromatium vinosum	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Bradyrhizobium lupini	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Anabaena cylindrica	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Azotobacter chroococcum	-	-
additional information	not established, whether the nitrogenase exists in vivo in a specific particle or whether the nitrogenase proteins are bound nonspecifically to the membranes of some cells	Corynebacterium flavescens	-	-

Metals/Ions

Metals/Ions	Comment	Organism
Mg2+	Mg2+ required for MgATP complex	Bradyrhizobium japonicum
Mg2+	Mg2+ required for MgATP complex	Klebsiella pneumoniae
Mg2+	Mg2+ required for MgATP complex	Clostridium pasteurianum
Mg2+	Mg2+ required for MgATP complex	Azotobacter vinelandii
Mg2+	Mg2+ required for MgATP complex	Paenibacillus polymyxa
Mg2+	Mg2+ required for MgATP complex	Rhodospirillum rubrum
Mg2+	Mg2+ required for MgATP complex	Allochromatium vinosum
Mg2+	Mg2+ required for MgATP complex	Bradyrhizobium lupini
Mg2+	Mg2+ required for MgATP complex	Anabaena cylindrica
Mg2+	Mg2+ required for MgATP complex	Azotobacter chroococcum
Mg2+	Mg2+ required for MgATP complex	Corynebacterium flavescens
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Bradyrhizobium japonicum
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Klebsiella pneumoniae
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Clostridium pasteurianum
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Azotobacter vinelandii
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Paenibacillus polymyxa
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Rhodospirillum rubrum
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Allochromatium vinosum
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Bradyrhizobium lupini
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Anabaena cylindrica
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Azotobacter chroococcum
additional information	other metal ions, e.g. Cu2+, Mg2+, Zn2+, Ca2+, at levels of 1-2 atoms per mol detected in the MoFe protein, no evidence for specific requirement, except for Mg2+ in MgATP complex, of any of these metals	Corynebacterium flavescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	Fe protein, ultracentrifugation	Clostridium pasteurianum
40000	74000	Fe protein: various methods, overview	Bradyrhizobium japonicum
40000	74000	Fe protein: various methods, overview	Klebsiella pneumoniae
40000	74000	Fe protein: various methods, overview	Clostridium pasteurianum
40000	74000	Fe protein: various methods, overview	Azotobacter vinelandii
40000	74000	Fe protein: various methods, overview	Bradyrhizobium lupini
51000	-	Fe protein, gel filtration	Bradyrhizobium japonicum
56000	-	Fe protein, gel filtration	Clostridium pasteurianum
62000	-	Fe protein, gel filtration	Klebsiella pneumoniae
64000	-	Fe protein, gel filtration	Azotobacter vinelandii
64000	-	Fe protein, gel filtration	Azotobacter chroococcum
65000	-	Fe protein, gel filtration	Bradyrhizobium lupini
68200	-	Fe protein, ultracentrifugation	Klebsiella pneumoniae
160000	-	MoFe protein, estimation from Mo content, ultracentrifugation	Allochromatium vinosum
160000	270000	MoFe protein: various methods, overview	Bradyrhizobium japonicum
160000	270000	MoFe protein: various methods, overview	Klebsiella pneumoniae
160000	270000	MoFe protein: various methods, overview	Clostridium pasteurianum
160000	270000	MoFe protein: various methods, overview	Azotobacter vinelandii
160000	270000	MoFe protein: various methods, overview	Allochromatium vinosum
160000	270000	MoFe protein: various methods, overview	Bradyrhizobium lupini
160000	270000	MoFe protein: various methods, overview	Azotobacter chroococcum
200000	-	-	Clostridium pasteurianum
200000	-	MoFe protein, ultracentrifugation	Bradyrhizobium japonicum
200000	-	MoFe protein, ultracentrifugation	Klebsiella pneumoniae
270000	-	MoFe protein, ultracentrifugation	Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Bradyrhizobium japonicum	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Klebsiella pneumoniae	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Clostridium pasteurianum	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Azotobacter vinelandii	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Paenibacillus polymyxa	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Rhodospirillum rubrum	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Allochromatium vinosum	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Bradyrhizobium lupini	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Anabaena cylindrica	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Azotobacter chroococcum	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	Corynebacterium flavescens	-	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Allochromatium vinosum	-	-	-
Anabaena cylindrica	-	-	-
Azotobacter chroococcum	-	-	-
Azotobacter vinelandii	-	-	-
Bradyrhizobium japonicum	-	-	-
Bradyrhizobium lupini	-	-	-
Clostridium pasteurianum	-	-	-
Corynebacterium flavescens	-	-	-
Klebsiella pneumoniae	-	-	-
Paenibacillus polymyxa	-	-	-
Rhodospirillum rubrum	-	-	-

Oxidation Stability

Oxidation Stability	Organism
extreme sensitivity to O2	Bradyrhizobium japonicum
extreme sensitivity to O2	Klebsiella pneumoniae
extreme sensitivity to O2	Clostridium pasteurianum
extreme sensitivity to O2	Azotobacter vinelandii
extreme sensitivity to O2	Paenibacillus polymyxa
extreme sensitivity to O2	Rhodospirillum rubrum
extreme sensitivity to O2	Allochromatium vinosum
extreme sensitivity to O2	Bradyrhizobium lupini
extreme sensitivity to O2	Anabaena cylindrica
extreme sensitivity to O2	Azotobacter chroococcum
extreme sensitivity to O2	Corynebacterium flavescens

Reaction

Reaction	Comment	Organism	Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Bradyrhizobium japonicum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Klebsiella pneumoniae	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Clostridium pasteurianum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Azotobacter vinelandii	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Paenibacillus polymyxa	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Rhodospirillum rubrum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Allochromatium vinosum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Bradyrhizobium lupini	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Anabaena cylindrica	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Azotobacter chroococcum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	catalytic mechanism	Corynebacterium flavescens	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Bradyrhizobium japonicum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Klebsiella pneumoniae	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Clostridium pasteurianum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Azotobacter vinelandii	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Paenibacillus polymyxa	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Rhodospirillum rubrum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Allochromatium vinosum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Bradyrhizobium lupini	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Anabaena cylindrica	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Azotobacter chroococcum	a
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein	Corynebacterium flavescens	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Bradyrhizobium japonicum
additional information	-	-	Klebsiella pneumoniae
additional information	-	-	Clostridium pasteurianum
additional information	-	-	Azotobacter vinelandii
additional information	-	-	Paenibacillus polymyxa
additional information	-	-	Rhodospirillum rubrum
additional information	-	-	Allochromatium vinosum
additional information	-	-	Bradyrhizobium lupini
additional information	-	-	Anabaena cylindrica
additional information	-	-	Azotobacter chroococcum
additional information	-	-	Corynebacterium flavescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Bradyrhizobium japonicum	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Klebsiella pneumoniae	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Clostridium pasteurianum	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Azotobacter vinelandii	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Paenibacillus polymyxa	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Rhodospirillum rubrum	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Allochromatium vinosum	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Bradyrhizobium lupini	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Anabaena cylindrica	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Azotobacter chroococcum	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O	-	Corynebacterium flavescens	8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Bradyrhizobium japonicum
dimer	-	Klebsiella pneumoniae
dimer	-	Clostridium pasteurianum
dimer	-	Azotobacter vinelandii
dimer	-	Paenibacillus polymyxa
dimer	-	Rhodospirillum rubrum
dimer	-	Allochromatium vinosum
dimer	-	Bradyrhizobium lupini
dimer	-	Anabaena cylindrica
dimer	-	Azotobacter chroococcum
dimer	-	Corynebacterium flavescens
tetramer	-	Bradyrhizobium japonicum
tetramer	-	Klebsiella pneumoniae
tetramer	-	Clostridium pasteurianum
tetramer	-	Azotobacter vinelandii
tetramer	-	Paenibacillus polymyxa
tetramer	-	Rhodospirillum rubrum
tetramer	-	Allochromatium vinosum
tetramer	-	Bradyrhizobium lupini
tetramer	-	Anabaena cylindrica
tetramer	-	Azotobacter chroococcum
tetramer	-	Corynebacterium flavescens