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Literature summary for 1.18.6.1 extracted from

  • Palmer, G.
    Iron-sulfur proteins (1975), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 12, 1-56.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
O2
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.2 gatom per mol of MoFe protein Klebsiella pneumoniae
Cu2+ 1.4 gatom per mol of MoFe protein Klebsiella pneumoniae
Iron 4 atoms of iron per molecule of Fe protein Klebsiella pneumoniae
Iron 17.5 gatom per mol of MoFe protein Klebsiella pneumoniae
Mg2+ 1.8 gatom per mol of MoFe protein Klebsiella pneumoniae
Mg2+ Mg2+ required for MgATP complex Klebsiella pneumoniae
Molybdenum 1 gatom per mol of MoFe protein Klebsiella pneumoniae
Zn2+ 0.8 gatom per mol of MoFe protein Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
62000
-
Fe protein, gel filtration Klebsiella pneumoniae
220000
-
MoFe protein, gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Oxidation Stability

Oxidation Stability Organism
extreme O2 lability, t1/2: 10 min, MoFe protein 45 s, Fe protein Klebsiella pneumoniae

Reaction

Reaction Comment Organism Reaction ID
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?