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Literature summary for 1.18.6.1 extracted from
- Purification aof nitrogenase from Clostridium pasteurianum (1972), Methods Enzymol., 24B, 446-456.
General Stability
| General Stability | Organism |
|---|---|
| Fe protein is salt sensitive | Clostridium pasteurianum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Clostridium pasteurianum |  |
| Molybdenum | - |
Clostridium pasteurianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Clostridium pasteurianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | Clostridium pasteurianum | - |
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium pasteurianum | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| Fe protein is very O2 sensitive | Clostridium pasteurianum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strict anaerobic conditions | Clostridium pasteurianum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Clostridium pasteurianum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.2 | - |
purified MoFe protein | Clostridium pasteurianum |
| 1.7 | 2.2 | purified Fe protein | Clostridium pasteurianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Clostridium pasteurianum | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Clostridium pasteurianum |
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Release 2023.1 (January 2023)
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