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Literature summary for 1.18.6.1 extracted from

  • Klassen, G.; de Oliveira Pedrosa, F.; de Souza, E.M.; Yates, M.G.; Rigo, L.U.
    Nitrogenase activity of Herbaspirillum seropedicae grown under low iron levels requires the products of nifXorf1 genes (2003), FEMS Microbiol. Lett., 224, 255-259.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genetic mapping Herbaspirillum seropedicae

Protein Variants

Protein Variants Comment Organism
additional information strains mutated in the nifX or orf1 genes show 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum, respectively Herbaspirillum seropedicae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron dependent on, the enzyme complex contains a molybdenum-iron, a tetramer with 2 different subunits and 4 organo-metallic clusters, i.e. 2 iron-molybdenum cofactors and 2 P-clusters, the enzyme complex contains also a homodimeric iron protein encoded by the nifH gene Herbaspirillum seropedicae
Molybdenum dependent on, the enzyme complex contains a molybdenum-iron, a tetramer with 2 different subunits and 4 organo-metallic clusters, i.e. 2 iron-molybdenum cofactors and 2 P-clusters Herbaspirillum seropedicae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Herbaspirillum seropedicae gene nifX and the contigous gene orf1 are essential for maximum nitrogen fixation under iron limitation and are probably involved in synthesis of nitrogenase iron or iron-molybdenum clusters ?
-
?
reduced ferredoxin + H+ + N2 + ATP + H2O Herbaspirillum seropedicae
-
oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Herbaspirillum seropedicae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + ?
-
Herbaspirillum seropedicae ethylene + ?
-
?
additional information gene nifX and the contigous gene orf1 are essential for maximum nitrogen fixation under iron limitation and are probably involved in synthesis of nitrogenase iron or iron-molybdenum clusters Herbaspirillum seropedicae ?
-
?
reduced ferredoxin + H+ + N2 + ATP + H2O
-
Herbaspirillum seropedicae oxidized ferredoxin + H2 + NH3 + ADP + phosphate
-
ir

Cofactor

Cofactor Comment Organism Structure
ATP
-
Herbaspirillum seropedicae
Ferredoxin
-
Herbaspirillum seropedicae