Cloned (Comment) | Organism |
---|---|
in Escherichia coli BL21 cells | Acinetobacter sp. HBS-2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00049 | - |
propionaldehyde | - |
Acinetobacter sp. HBS-2 | |
0.00212 | - |
formaldehyde | - |
Acinetobacter sp. HBS-2 | |
0.01518 | - |
acetaldehyde | - |
Acinetobacter sp. HBS-2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activity of the enzyme is elevated | Acinetobacter sp. HBS-2 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
SDS-PAGE | Acinetobacter sp. HBS-2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | Acinetobacter sp. HBS-2 | - |
acetyl-CoA + NADH + H+ | - |
r | |
formaldehyde + CoA + NAD+ | Acinetobacter sp. HBS-2 | - |
formyl-CoA + NADH + H+ | - |
? | |
propionaldehyde + CoA + NAD+ | Acinetobacter sp. HBS-2 | - |
propionyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. HBS-2 | A5JT11 | - |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography by means of a Ni-Sepharose column | Acinetobacter sp. HBS-2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.6 | - |
recombinant enzyme | Acinetobacter sp. HBS-2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Acinetobacter sp. HBS-2 | acetyl-CoA + NADH + H+ | - |
r | |
formaldehyde + CoA + NAD+ | - |
Acinetobacter sp. HBS-2 | formyl-CoA + NADH + H+ | - |
? | |
propionaldehyde + CoA + NAD+ | - |
Acinetobacter sp. HBS-2 | propionyl-CoA + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Acinetobacter sp. HBS-2 |
Synonyms | Comment | Organism |
---|---|---|
acetaldehyde dehydrogenase | - |
Acinetobacter sp. HBS-2 |
aldehyde dehydrogenase | - |
Acinetobacter sp. HBS-2 |
ALDH | - |
Acinetobacter sp. HBS-2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter sp. HBS-2 |