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Literature summary for 1.2.1.104 extracted from

  • Rapp, B.J.; Miernyk, J.A.; Randall, D.D.
    Pyruvate dehydrogenase complexes from Ricinus communis endosperm (1987), J. Plant Physiol., 127, 293-306.
No PubMed abstract available
Search for more literature for 1.2.1.104

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Ricinus communis
NADH
-
 Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 coenzyme A assay with whole enzyme complex Ricinus communis
0.051
-
 NAD+ assay with whole enzyme complex Ricinus communis
0.09
-
 pyruvate assay with whole enzyme complex Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-
mitochondrion
-
 Ricinus communis 5739
-
plastid distinct from mitochondrial enzyme, different subunit composition Ricinus communis 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Bos taurus
Ca2+ can replace Mg2+ Ricinus communis
Mg2+
-
 Ricinus communis
Mn2+ can replace Mg2+ Ricinus communis

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Ricinus communis
-
 var. N5
-

Purification (Commentary)

Purification (Comment) Organism
pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
 Ricinus communis acetyl-CoA + CO2 + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Ricinus communis

Cofactor

Cofactor Comment Organism Structure
additional information CoA and NAD+ required by pyruvate dehydrogenase complex Ricinus communis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
 NADH competitive inhibition vs. NAD+, whole enzyme complex Ricinus communis
0.02
-
 acetyl-CoA competitive inhibition vs. CoA, whole enzyme complex Ricinus communis