Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | 1-10 mM, slight activation | [Bacillus] caldolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | 5 mM, about 50% inhibition after 3 h | Geobacillus stearothermophilus | |
MgATP2- | 5 mM, about 70% inhibition after 3 h | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
42000 | - |
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
100000 | - |
x * 100000, SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
[Bacillus] caldolyticus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | not regulated by phosphorylation/dephosphorylation | Geobacillus stearothermophilus |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
pyruvate dehydrogenase can be resolved from complex by incubation with 8 M urea, 4 M guanidinium chloride, 100 mM glycine/NaOH, pH 9.0 followed by gel filtration | Thermus thermophilus |
Renatured (Comment) | Organism |
---|---|
no reconstitution of whole complex, pyruvate dehydrogenase subunit regains some activity after separation from complex in the presence of chaperonins and ATP/MgCl2 | Geobacillus stearothermophilus |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Thermus thermophilus |
tetramer | alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pyruvate dehydrogenase complex, no loss of activity after 50 min | Geobacillus stearothermophilus |
72 | - |
pyruvate dehydrogenase, complete loss of activity | Geobacillus stearothermophilus |
84 | - |
pyruvate dehydrogenase | [Bacillus] caldolyticus |