Application | Comment | Organism |
---|---|---|
synthesis | exchange of the native Corynebacterium glutamicum promoter of the AceE gene, with mutated DapA promoter variants leads to a series of strains with gradually reduced growth rates and pyruvate dehydrogenase complex activities. Upon overexpression of the L-valine biosynthetic genes IlvBNCE, all strains produce L-valine. Additional deletion of the Pqo and Ppc genes, encoding pyruvate:quinone oxidoreductase and phosphoenolpyruvate carboxylase enables production of up to 738mM (i.e., 86.5 g/liter). Inactivation of the transaminase B gene (IlvE) and overexpression of IlvBNCD instead of ilvBNCE transform the L-valine-producing strain into a 2-ketoisovalerate producer, excreting up to 303mM (35 g/liter) 2-ketoisovalerate. The replacement of the AceE promoter by the DapA-A16 promoter improves the production by 100% and 44%, respectively | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NNF6 | Q8NNF6 i.e. E1 component AceE, cf. EC 1.2.4.1 | - |
Synonyms | Comment | Organism |
---|---|---|
aceE | - |
Corynebacterium glutamicum |