Literature summary for 1.2.1.105 extracted from
Shiio, I.; Ujigawa-Takeda, U.
Presence and regulation of alphe-ketoglutarate dehydrogenase complex in a glutamate-producing bacterium Brevibacterium flavum (1980), Agric. Biol. Chem., 44, 1897-1904.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
acetyl-CoA |
activates, 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Cys |
activates, 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
General Stability
General Stability |
Organism |
2-oxoglutarate dehydrogenase complex is stabilized by glycerol, Mg2+ and thiamine diphosphate |
[Brevibacterium] flavum |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
cis-aconitate |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
K+ |
50 mM KCl, 63% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Na+ |
50 mM NaCl, 34% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Na2HPO4 |
50 mM KCl, 38% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Na2SO4 |
50 mM KCl, 4.5% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
NADH |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
NADPH |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
NH4+ |
50 mM NH4Cl, 21% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
oxalacetate |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
pyruvate |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
succinyl-CoA |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Tris-HCl |
50 mM, 26% inhibition of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.076 |
- |
2-oxoglutarate |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
0.08 |
- |
2-oxoglutarate |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
required, 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
additional information |
- |
7000000 Da is the MW of the 2-oxoglutarate dehydrogenase complex, gel filtration |
[Brevibacterium] flavum |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
2-oxoglutarate + lipoamide |
[Brevibacterium] flavum |
addition of glutamate stimulates the synthesis of the 2-oxoglutarate dehydrogenase complex |
S-succinyldihydrolipoamide + CO2 |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
[Brevibacterium] flavum |
- |
- |
- |
Reaction
Reaction |
Comment |
Organism |
Reaction ID |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 |
2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) |
[Brevibacterium] flavum |
|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 |
the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH |
[Brevibacterium] flavum |
|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 |
mechanism of the enzyme complex is bi bi uni uni ping pong |
[Brevibacterium] flavum |
|
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
2-oxoglutarate + lipoamide |
- |
[Brevibacterium] flavum |
S-succinyldihydrolipoamide + CO2 |
- |
? |
|
2-oxoglutarate + lipoamide |
addition of glutamate stimulates the synthesis of the 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
S-succinyldihydrolipoamide + CO2 |
- |
? |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
7.7 |
2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6.5 |
8.5 |
pH 6.5: about 60% of maximal activity, pH 8.5: about 30% of maximal activity, 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
thiamine diphosphate |
required , 2-oxoglutarate dehydrogenase complex |
[Brevibacterium] flavum |
|