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Literature summary for 1.2.1.105 extracted from

  • Hirabayashi, T.; Harada, T.
    Isolation and properties of alpha-ketoglutarate dehydrogenase complex from bakers yeast (Saccharomyces cerevisiae) (1971), Biochem. Biophys. Res. Commun., 45, 1369-1375.
    View publication on PubMed
Search for more literature for 1.2.1.105

Activating Compound

Activating Compound Comment Organism Structure
Cys activates, 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 2-oxoglutarate
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of the 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + lipoamide
-
 Saccharomyces cerevisiae S-succinyldihydrolipoamide + CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required , 2-oxoglutarate dehydrogenase complex Saccharomyces cerevisiae