KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
2-oxoglutarate | pH 7.4, 30°C | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Neurospora crassa | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification procedure for the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes from mitochondria. After fractionated precipitations with polyethylene glycol, elimination of thiol proteins, and gel-filtration chromatography, the resulting preparations contain both activities. Covalent chromatography on thiol-activated Sepharose CL-4B allows the specific binding of the 2-oxoglutarate dehydrogenase complex activity in the presence of 2-oxoglutarate, the pyruvate dehydrogenase complex activity is retained in the presence of pyruvate | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + NAD+ | - |
Neurospora crassa | succinyl-CoA + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 160000, x * 56200, x * 55600, x * 52600, SDS-PAGE | Neurospora crassa |