Literature summary for 1.2.1.107 extracted from

Oshima, T.; Fujita, S.; Imahori, K.
[58] Glyceraldehyde-3-phosphate dehydrogenase from Thermus thermophilus (1982), Methods Enzymol., 89, 335-340 .

No PubMed abstract available

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Activating Compound

Activating Compound	Comment	Organism	Structure
(NH4)2SO4	the enzyme activity is increased about 10fold in the presence of 0.045 M (NH4)2SO4	Thermus thermophilus
Na2SO4	-	Thermus thermophilus
NaCI	-	Thermus thermophilus
NH4Cl	25fold activation in the presence of 0.1 M	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	strong inhibitor	Thermus thermophilus
Cu2+	strong inhibitor	Thermus thermophilus
Hg2+	strong inhibitor	Thermus thermophilus
Monoiodoacetamide	-	Thermus thermophilus
monoiodoacetate	potent inhibitor	Thermus thermophilus
Sodium tetrathionate	potent inhibitor	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	NAD+	at pH 8.3 and 30°C	Thermus thermophilus
0.3	-	D-glyceraldehyde 3-phosphate	at pH 8.3 and 30°C	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cs+	activates	Thermus thermophilus
K+	activates	Thermus thermophilus
Li+	activates	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + arsenate + NAD+	Thermus thermophilus	-	1-arsono-3-phospho-D-glycerate + NADH + H+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and DEAE-Sephadex A-50 gel filtration	Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.11	-	crude extract, at pH 8.3 and 30°C	Thermus thermophilus
5.4	-	purified enzyme, at pH 8.3 and 30°C	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate + arsenate + NAD+	-	Thermus thermophilus	1-arsono-3-phospho-D-glycerate + NADH + H+	-	ir

Subunits

Subunits	Comment	Organism
homotetramer	4 * 33500, SDS-PAGE	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
G3P dehydrogenase	-	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	-	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermus thermophilus

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Release 2023.1 (January 2023)