KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NADP+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
156000 | - |
sedimentation equilibrium, meniscus depletion technique | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Saccharomyces cerevisiae | synthesis of threonine | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200 | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | synthesis of threonine | Saccharomyces cerevisiae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Saccharomyces cerevisiae | L-4-aspartyl phosphate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharomyces cerevisiae |