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Literature summary for 1.2.1.11 extracted from

  • Moore, R.A.; Bocik, W.E.; Viola, R.E.
    Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms (2002), Protein Expr. Purif., 25, 189-194.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI Helicobacter pylori
expressed in Escherichia coli Haemophilus influenzae
expressed in Escherichia coli Pseudomonas aeruginosa
expressed in Escherichia coli, the genome contains two genes for the enzyme Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
0.13
-
NADP+
-
Pseudomonas aeruginosa
0.15
-
NADP+
-
Haemophilus influenzae
0.16
-
L-aspartate 4-semialdehyde gene II Vibrio cholerae serotype O1
0.19
-
L-aspartate 4-semialdehyde gene I Vibrio cholerae serotype O1
0.24
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae
0.32
-
NADP+ gene I Vibrio cholerae serotype O1
0.36
-
NADP+ gene II Vibrio cholerae serotype O1
1.1
-
phosphate gene I Vibrio cholerae serotype O1
1.5
-
phosphate
-
Pseudomonas aeruginosa
1.6
-
phosphate
-
Haemophilus influenzae
22
-
phosphate gene II Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH Haemophilus influenzae
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH Vibrio cholerae serotype O1
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH Pseudomonas aeruginosa
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH Helicobacter pylori
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-
Helicobacter pylori O25801
-
-
Pseudomonas aeruginosa Q51344
-
-
Vibrio cholerae P23247
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Helicobacter pylori
using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A Haemophilus influenzae
using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II Vibrio cholerae serotype O1
using column chromatography on Q Sepharose XL and phenyl-Sepharose Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Haemophilus influenzae
additional information
-
-
Vibrio cholerae serotype O1
additional information
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH
-
Haemophilus influenzae L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH
-
Vibrio cholerae serotype O1 L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH
-
Pseudomonas aeruginosa L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-4-aspartyl phosphate + NADPH
-
Helicobacter pylori L-aspartate 4-semialdehyde + phosphate + NADP+
-
?
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Haemophilus influenzae L-4-aspartyl phosphate + NADPH
-
r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Vibrio cholerae serotype O1 L-4-aspartyl phosphate + NADPH
-
r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Pseudomonas aeruginosa L-4-aspartyl phosphate + NADPH
-
r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
Helicobacter pylori L-4-aspartyl phosphate + NADPH
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58
-
L-aspartate 4-semialdehyde gene II Vibrio cholerae serotype O1
120
-
L-aspartate 4-semialdehyde gene I Vibrio cholerae serotype O1
160
-
L-aspartate 4-semialdehyde
-
Pseudomonas aeruginosa
330
-
L-aspartate 4-semialdehyde
-
Haemophilus influenzae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Haemophilus influenzae
NADP+
-
Vibrio cholerae serotype O1
NADP+
-
Pseudomonas aeruginosa
NADP+
-
Helicobacter pylori