Cloned (Comment) | Organism |
---|---|
expressed as inclusion bodies with an N-terminal hexahistidine tag in pET28b vector or subcloned into pET41 vector with NdeI/SalI | Helicobacter pylori |
expressed in Escherichia coli | Haemophilus influenzae |
expressed in Escherichia coli | Pseudomonas aeruginosa |
expressed in Escherichia coli, the genome contains two genes for the enzyme | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-aspartate 4-semialdehyde | - |
Pseudomonas aeruginosa | |
0.13 | - |
NADP+ | - |
Pseudomonas aeruginosa | |
0.15 | - |
NADP+ | - |
Haemophilus influenzae | |
0.16 | - |
L-aspartate 4-semialdehyde | gene II | Vibrio cholerae serotype O1 | |
0.19 | - |
L-aspartate 4-semialdehyde | gene I | Vibrio cholerae serotype O1 | |
0.24 | - |
L-aspartate 4-semialdehyde | - |
Haemophilus influenzae | |
0.32 | - |
NADP+ | gene I | Vibrio cholerae serotype O1 | |
0.36 | - |
NADP+ | gene II | Vibrio cholerae serotype O1 | |
1.1 | - |
phosphate | gene I | Vibrio cholerae serotype O1 | |
1.5 | - |
phosphate | - |
Pseudomonas aeruginosa | |
1.6 | - |
phosphate | - |
Haemophilus influenzae | |
22 | - |
phosphate | gene II | Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Haemophilus influenzae | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | Vibrio cholerae serotype O1 | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | Pseudomonas aeruginosa | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | Helicobacter pylori | - |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Helicobacter pylori | O25801 | - |
- |
Pseudomonas aeruginosa | Q51344 | - |
- |
Vibrio cholerae | P23247 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A | Haemophilus influenzae |
using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II | Vibrio cholerae serotype O1 |
using column chromatography on Q Sepharose XL and phenyl-Sepharose | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Haemophilus influenzae |
additional information | - |
- |
Vibrio cholerae serotype O1 |
additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | - |
Haemophilus influenzae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | - |
Vibrio cholerae serotype O1 | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | - |
Pseudomonas aeruginosa | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-4-aspartyl phosphate + NADPH | - |
Helicobacter pylori | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Haemophilus influenzae | L-4-aspartyl phosphate + NADPH | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Vibrio cholerae serotype O1 | L-4-aspartyl phosphate + NADPH | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Pseudomonas aeruginosa | L-4-aspartyl phosphate + NADPH | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Helicobacter pylori | L-4-aspartyl phosphate + NADPH | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
L-aspartate 4-semialdehyde | gene II | Vibrio cholerae serotype O1 | |
120 | - |
L-aspartate 4-semialdehyde | gene I | Vibrio cholerae serotype O1 | |
160 | - |
L-aspartate 4-semialdehyde | - |
Pseudomonas aeruginosa | |
330 | - |
L-aspartate 4-semialdehyde | - |
Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haemophilus influenzae | |
NADP+ | - |
Vibrio cholerae serotype O1 | |
NADP+ | - |
Pseudomonas aeruginosa | |
NADP+ | - |
Helicobacter pylori |