Crystallization (Comment) | Organism |
---|---|
about 10 mg/ml pure recombinant wild-type enzyme in 10 mM HEPES, pH 7.0, 1 mM EDTA, and 1 mM DTT, complexed with oxyanions arsenate or periodate, hanging drop vapour diffusion method, 20°C, against an equal volume of precipitant solution containing 22-24% PEG 4000, 0.2 M ammonium acetate, and Tris-HCl, pH 8.5, soaking of crystals before harvest in a solution containing 26% PEG3350, 0.2 M ammonium acetate, 100 mM periodate or arsenate,0.1 M Tris-HCl, pH 8.5, and 20% glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibitor binding structure and mechanism | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | Haemophilus influenzae | physiological forward reaction, reductive dephosphorylation in the aspartate biosynthetic pathway | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P44801 | gene asd | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH + H+ | catalytic mechanism, substrate recognition | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-4-aspartyl phosphate + NADPH | physiological forward reaction, reductive dephosphorylation in the aspartate biosynthetic pathway | Haemophilus influenzae | L-aspartate-4-semialdehyde + phosphate + NADP+ | - |
r | |
L-aspartate-4-semialdehyde + phosphate + NADP+ | formation of an acyl-enzyme intermediate | Haemophilus influenzae | L-4-aspartyl phosphate + NADPH | - |
r | |
additional information | oxyanion binding sites and structures with arsenate and periodate | Haemophilus influenzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASADH | - |
Haemophilus influenzae |
aspartate-beta-semialdehyde dehydrogenase | - |
Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haemophilus influenzae | |
NADPH | - |
Haemophilus influenzae |