Literature summary for 1.2.1.11 extracted from

Dahal, G.P.; Viola, R.E.
Structure of a fungal form of aspartate-semialdehyde dehydrogenase from Aspergillus fumigatus (2017), Acta Crystallogr. Sect. F, 73, 36-44 .

Application

Application	Comment	Organism
drug development	the enzyme is a validated target for antimicrobial drug design	Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment)	Organism
gene AFUA_3G06830, codon-optimized, recombinant expression of C-terminally His-tagged enzyme ASADH in Escherichia coli strain BL21(DE3)	Aspergillus fumigatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant C-terminally His-tagged enzyme, hanging drop vapour diffusion method, crystallization screening, mixing of 0.001 ml protein solution plus 0.001 ml reservoir solution containing 1.9 M ammonium sulfate, 100 mM Bis-Tris, pH 6.5, at 20°C, crystals are either soaked or cocrystallized with 5 mM NADP+ and with several moderate inhibitors that are identified from fragment library screening, crystal cryoprotection with reservoir solution containing 20% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 2.8-3.2 A resolution, modeling by molecular replacement using CaASADH, PDB ID 3hsk, as the search model	Aspergillus fumigatus

Crystallization (Comment)

Organism

purified recombinant C-terminally His-tagged enzyme, hanging drop vapour diffusion method, crystallization screening, mixing of 0.001 ml protein solution plus 0.001 ml reservoir solution containing 1.9 M ammonium sulfate, 100 mM Bis-Tris, pH 6.5, at 20°C, crystals are either soaked or cocrystallized with 5 mM NADP+ and with several moderate inhibitors that are identified from fragment library screening, crystal cryoprotection with reservoir solution containing 20% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 2.8-3.2 A resolution, modeling by molecular replacement using CaASADH, PDB ID 3hsk, as the search model

Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+	Aspergillus fumigatus	-	L-4-aspartyl phosphate + NADPH + H+	-	r
L-aspartate 4-semialdehyde + phosphate + NADP+	Aspergillus fumigatus ATCC MYA-4609	-	L-4-aspartyl phosphate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Aspergillus fumigatus	Q4WWR8	-	-
Aspergillus fumigatus ATCC MYA-4609	Q4WWR8	-	-
no activity in Homo sapiens	-	and other mammals	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His-tagged enzyme ASADH from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and dialysis	Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-aspartate 4-semialdehyde + phosphate + NADP+	-	Aspergillus fumigatus	L-4-aspartyl phosphate + NADPH + H+	-	r
L-aspartate 4-semialdehyde + phosphate + NADP+	-	Aspergillus fumigatus ATCC MYA-4609	L-4-aspartyl phosphate + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	enzyme AfASADH displays a crystallographic dimer	Aspergillus fumigatus
More	enzyme secondary structure, crystal structure analysis, detailed overview	Aspergillus fumigatus

Synonyms

Synonyms	Comment	Organism
AFUA_3G06830	-	Aspergillus fumigatus
ASADH	-	Aspergillus fumigatus

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Aspergillus fumigatus
NADPH	-	Aspergillus fumigatus

General Information

General Information	Comment	Organism
metabolism	aspartate-semialdehyde dehydrogenase catalyzes the reductive dephosphorylation of the substrate beta-aspartyl phosphate into aspartate semialdehyde, a key intermediate in the aspartate biosynthetic pathway and functions at a critical junction in the aspartate biosynthetic pathway	Aspergillus fumigatus
additional information	active site structure analysis and comparison, detailed overview	Aspergillus fumigatus
physiological function	the enzyme catalyzes the NADPH-dependent reductive dephosphorylation of 4-aspartyl phosphate to produce the key intermediate aspartate semialdehyde	Aspergillus fumigatus