Literature summary for 1.2.1.12 extracted from

Wrba, A.; Schweiger, A.; Schultes, V.; Jaenicke, R.; Zavodszky, P.
Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima (1990), Biochemistry, 29, 7584-7592.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	4 * 37000, SDS-PAGE	Thermotoga maritima
148000	-	equilibrium sedimentation	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
44	-	-	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-phospho-D-glyceroyl phosphate + NADH	-	Thermotoga maritima	D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	-	Thermotoga maritima	3-phospho-D-glyceroyl phosphate + NADH	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 37000, SDS-PAGE	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	incubation at 70-85°C results in an apparent activation	Thermotoga maritima
95	-	t1/2 at pH 5.0: 25 min, t1/2 at pH 6.0: more than 120 min, t1/2 at pH 7.5: 50 min	Thermotoga maritima
100	-	t1/2: above 120 min at pH 6	Thermotoga maritima
109	-	transition temperature	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9	oxidation of D-glyceraldehyde 3-phosphate, at 40°C	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10	pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity, oxidation of D-glyceraldehyde 3-phosphate, at 40°C	Thermotoga maritima

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermotoga maritima
NADH	cofactor	Thermotoga maritima

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Release 2023.1 (January 2023)